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GENETIC TRANSFORMATION OF PSY INTO SOYBEAN BY PARTICLE BOMBARDMENT(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年02期
Page:
137-140
Research Field:
Publishing date:

Info

Title:
GENETIC TRANSFORMATION OF PSY INTO SOYBEAN BY PARTICLE BOMBARDMENT
Author(s):
Gong Xuechen 1 　 Ji Jing 2 　 Wang Gang3　 Wang Ping 1
(1. Hebei North University , Zhangjiakou , 075131 ;2. Tianjin University , Tianjin, 300072;3. Huaihai Institute of Technology ,Marine School , Lianyungang ,222005)
Keywords:
Particle bombardment ; PSY gene ; Soybean; Somatic embryogenesis; Genetic Transformation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.02.0137
Abstract:
　 Using somatic embryogenesis of three varieties of soybean as acceptor , PSY gene were trans-formed into soybean via particle bombardment. The results showed:the sensitivity of somatic embryo ofsoybean with different genotypes to resistance selective marker(Hyg) is significantly different ;high os-motic pressure medium with 0. 4mol /L mannitol was beneficial for the transformation of target gene. 10resistant regenerated plants were obtained from the three varieties, 4 plants gave positive reaction in PCRand PCR- Southern analysis from the gene transferred plants ,indicating that PSY has been transferred intogenome of soybean.

References:

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Last Update: 2016-12-12