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THE DETERMINATION OF VITAMIN E USING HPLC AND THE LINEAR REGRESSION OFTHE CONTENTS OF VITAMIN E AND OIL IN SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年02期
Page:
113-117
Research Field:
Publishing date:

Info

Title:
THE DETERMINATION OF VITAMIN E USING HPLC AND THE LINEAR REGRESSION OFTHE CONTENTS OF VITAMIN E AND OIL IN SOYBEAN
Author(s):
Wang Li 1 　 Song Zhifeng1　 Ji Feng 1 　 Jin Weidong 1 　 Huang Huang 1Yu Zhijing 2 　 Tang Xiaobo 3
(1. Soybean Research Center of J ilin Academy of Agricultural Sciences ,Gongzhuling 136100;2. Graduate School of Jilin Agricultural University , Changchun 130118;3. Tilin City Academy of Agricultyral Science , Charychun 132101)
Keywords:
HPLC ; Soybean ; Vitamin E ; Oil ; Linear regression
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.02.0113
Abstract:
T he contents of the vitamin E in 1000 soybean occessions were determined by high performanceliquid chromatography with fluorescence detection (Ex =295nm , Em =330nm). The isomers of vitamin Ewere determined by the standards α-、β-、γ-、δ-tocopherols and the internal standard of the tocol. The vita-min E contents was 0. 05～ 0. 34g /mg in wild soybean resources, 0. 10～ 0. 38g /mg in cultivated soybean re-sources, respectively. The average recoveries of α-、β-、γ-、δ-tocopherol were 101. 10 %, 97. 50%, 96. 94%and 96. 30%, respectively. Meanwhile , the oil contents of soybean resources were determined by nationalstandard method (GB 2906～ 82). Their range were 3. 01%～ 20. 60%in wild soybean resources,8. 00 %～23. 59%in cultivated soybean resources, respectively. The positive correlation between the contents of vi-tamin E and oil was confirmed by the analysis of linear regression.

References:

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Last Update: 2016-12-12