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STUDY ON PREPARATION OF ISOFLAVONES AGLYCON(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年01期
Page:
94-96
Research Field:
Publishing date:

Info

Title:
STUDY ON PREPARATION OF ISOFLAVONES AGLYCON
Author(s):
Wang Lu 1 　 Li Yuhou 2 　 Sun Liyan 1 　 Han Jun 3 　Cui Hongbin 1
(1. Harbin Medical University , Harbin , 150086;2. Nutrition Institute of Heilongjiang ProvinceHospital , Harbin ,150036;3. Harbin Gene - tech Biochip Development Inc. , Ltd , Harbin, 150001)
Keywords:
Aglycon; Isoflavones; Preparation ; HPLC
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.01.0094
Abstract:
By orthogonal experimental methodology , the optimum parameters of acid hydrolysis for agly-con including Genistein were established, as follows:concentration of ethanol of acid, 2. 5 mol /L ;temper-ature, 90 ℃;and time 3h. According to solubility and polarity the Genistein of 48. 6%purity was preparedwith macro - pore resin AB - 8 desorping of terraced concentration(water →30%ethanol →80 %ethanol)and thin layed which the mixture of chloroform :methanol ∶20%acetic acid(7∶3∶1)was mobile phase.

References:

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Last Update: 2016-12-12