|Table of Contents|

MAPPING QUANTITATIVE TRAIT LOCI INFLUENCING TOLERANCE TOPHYTOPHTHORA ROOTROT IN SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年01期
Page:
23-27
Research Field:
Publishing date:

Info

Title:
MAPPING QUANTITATIVE TRAIT LOCI INFLUENCING TOLERANCE TOPHYTOPHTHORA ROOTROT IN SOYBEAN
Author(s):
Han Yingpeng1  Li Wenbin 1   Kangfu Yu 2   Terry R. Anderson 2   Vaino Poysa 2Wen Jingzhi3  Gao Jiguo4
(1.Soybean Research Institute , Northeast Agricultural University , Harbin 150030;2. Agriculture andAgri-food Canada , Greenhouse and Processing Crop Research Center , Canada;3.College of Li f eSciences, Northeast Agricultural University , Harbin 150030;4.Department of Plant Protection ,Agricultural college , Northeast Agricultural University , Harbin 150030)
Keywords:
Soybean Phytophthora root rot QTLsTolerance
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.01.0023
Abstract:
  T he purpose of this research is to identify quantitative trait loci (QT Ls) on the linkage groupswhich are associated with Soybean phytophthora root rot (PRR), in a population from a cross betweenConrad , a soybean cultivar tolerance to PRR in the field, and OX760, a breeding lines susceptible to phy-thora. DNA amplified via polymerase chain reaction using simple sequence repeat (SSR)marker and Ran-dom Amplified Polymorphic DNA (RAPD)marker. Based on the data of PRR at two locations , Woodslee(2000 and 2001 ) ,Weaver (2000) , and the two locations, two years average loss,four markers OPL18 -800bp, OPN03- 600bp;Satt536 , Satt463 , from two linkage groups , MLGD1b+W , MLGM ,were sig-nificantly associated with PRR . Each marker explained 13. 34%to 22. 31 %of phenotypic variance. T hreeputative QTLs :Q P - 1(OPL18- 800bp)Q P - 2 (OPN03 - 600bp) , Q P - 3 (Satt536 and Satt463 )cor-responding to these four markers , were designed. Multiple QT L analysis(Mapmaker / QTL1. 1)showedQP1 and Q P - 2 jointly explained 44. 5%of the phenotypic variance for the two locations ,two years aver-age loss . Q P - 3 explained 15. 2% of the phenotypic variance in Woodslee for 2001. QP - 1 explained21. 55%of that in Woodslee for 2000, 16. 71%of that in Wever for 2000, 22. 31%of that for the two loca-tions, two years average loss. Few QTLs were detected to be stable across most environments, which wasuseful for marker assisted-breeding.

References:

1  Schmitthenner, A. F. Promblems and progress in control of Phy-26                 大  豆  科  学                          1 期tophtho ra root rot of soy bean[ J] . Plant Dis, 1985, 69:362 - 368.

2 Fairs M. A. , and F. E. Sabo. T he systematics of Phytophthorasojae and P. megasperma [ J] . Can. J. Bot, 1989, 67:1442.
3   Ward E. W. B. , T he interaction of soya beans with Phytophthoramegasperma f. sp. Gly cinea :pathogeicity . Biological control ofsoilbrone plant pathogens[ M] . Edit by Hornby B. CAB Interna-tional, Wallingford, England. 1990, 3111- 327.
4 Anderson T . R. R. I. Buzzell . Divesity and frequence of races ofPhytophthora sojae f. sp. glycinea in soybean field in Essex Coun-ty , Ontario, 1980~ 1989[ J] . Plant Disease, 1992, 67:587 - 589.
5  M organ F. L. New Physiologic race of Phytophthora megaspermaVar. soje [ J] . Phy topatology , 1965, 55:1277 - 1279.
6  Leiz R. A. , Harman G. L. Races of Phytophthora sojae on Soy-bean in Illinois[ J] . Plant Disease , 2000, 84:487.
7  Drenth A , Whisson S.C. , MacLean D. J. , et al. The evolution ofPhytophthora sojae in Australia[ J] . Phytopathology , 1996, 86(2):163 - 169.
8  Schafer J. F. Toleranceto plant disease. Annual Rev[ J] . Phytopa-thology , 1971, 9:235 - 252.
9   Thomison P. R. , T homas , W. J. Kenwo rth. Evidence of pathogenspecify in tolerance of soybean cultivars to phytophthora rot[ J] .Crop Sci . , 1988, 28:714- 715.
10 Fehr, W. R. , S. R. , et al, Registration of Conrad soybean[ J] . CropSci . , 1989, 29:830.
11 Buzzel, R. I. , T. R. Anderson. Plant loss response of soybean culti-vars to Phytophthora megasperma f. sp. glycinea under field co-dotions[ J] . Plant Disease, 1982, 66:1146 - 1148.
12 Yu K ,S. J. , Park, V. Poysa. Abundance and variation of microsal-lite DNA sequences in beans(Phaseolus and Vigna)[ J] .Genome ,1999, 42:27 - 34.
13 Bassam, B. J. , G. Caetano - Anolles, P. M . Gresshoff . Fast andsensitive silver staining of DNA in polyacrylamide gels[ J] . AnalBiochem, 1991, 196:80 - 83.
14 T rigiano , R. N. , G. Caetano - Anolles. Laboratory exercises onDNA amplification fingerprinting for evaluating the molecular di-versity of horticultural species[ J] . Hort Technol, 1998, 8:413 -423.
15 LanderE.S. , Green, J. Abrahamson , A. Barlow , et al. MapMaker:An interactive computer package for constructing primary geneticlinkage maps of experimental and natural populations[ J] . Genom-ics, 1987, 1:174 - 181.
16 Cregan P. B. , T. Jarvik. An integ tated genetic linkage map of thesoybean genome[ J] . Crop Sci. , 1999, 39:1464 - 1490.
17 Arahana V. S. , G. L. Graef , J. E. Specht , et al. Identification ofQTLs for resistance to Scleritinia sclerotiorum in soybean[ J] .Crop Sci . , 2001, 41:180 - 188.

Memo

Memo:
-
Last Update: 2016-12-12