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The Content Variation of 7S ,11S Globulins andTheir Subunits of Seed Storage Protein of706 Chinese Soybean Germplasm*(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年01期
Page:
11-17
Research Field:
Publishing date:

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Title:
The Content Variation of 7S ,11S Globulins andTheir Subunits of Seed Storage Protein of706 Chinese Soybean Germplasm*
Author(s):
Ma Hao -Wang Xiansheng -Liu Chun -Yu Tian -Hao XiaoyanGao Wenrui -He Xiaoling
(State key Laboratory of Crop Genetics and Germplasm Enhancement , National Center for SoybeanImprovement , Ministry of Agriculture, Nanjing Agricultural University , Nanjing , 210095)
Keywords:
Glycine max (L. ) Merrill ; Germplasm ; SDS - PAGE; 11S ;  7S globulin and theirsubunits ; Relative content
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.01.0011
Abstract:
　T he contents of 7S and 11S globulins and their respective subunits, and the 11S /7S ra-tios were tightly associated with the nutrition and processing properties of soybean protein. Forimproving the nutritional qualities and the functional properties of soybean seed proteins, obtai-ning germplasm with different contents of 7S and 11S globulins and their respective subunits anddifferent ratios of the 11S/7S is a desirable objective. 706 accessions from Chinese germplasmwere investigated by Sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS - PAGE).The results showed that 7S globulin was divided as three subunits(α’,α,β), and 11S globulin wasdivided into three bands which were represented as A 3 , A 1 aA 1 bA 2 A 4 (Acid subunits) andB 1 aB 1 bB 2 B 3 B 4 (Basic subunits), respectively. There were great genetic diversity of the relativecontents of 11S , 7S globulins and their subunits in 706 Chinese soybean germplasm. Remarkablynegative correlation between the relative contents of 7S and 11S globulins (r =- 1. 00, P <0. 01)existed. The average and variation range of the relative content of 7S , 11S fraction of 603 Chineselandraces and 103 cultivars were 40. 00%,20. 58%～ 56. 65 %, 60. 00%, 43. 35%～ 79. 42% and38. 21%, 30. 33%～ 52. 67%, 61. 79 %, 47. 33%～ 69. 67%, respectively. The average and varia-tion range of the 11S /7S ratios of 603 Chinese landraces and 103 cultivars were 1. 54, 0. 77～ 3. 86and 1. 65, 0. 90～ 2. 30, respectively. 63 accessions screened from 706 germplasm showed poly-morphism of band type for the subunit relative contents.

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Last Update: 2016-12-12