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DETECTION OF BEAN POD MOTTLE VIRUS BY RT- PCR(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2005年04期
Page:
317-319
Research Field:
Publishing date:

Info

Title:
DETECTION OF BEAN POD MOTTLE VIRUS BY RT- PCR
Author(s):
Wei Meisheng1　 Xiang Ning1　 Zhang Chunquan2　 Said A. Ghabrial2
(1. Institute of Animal and Plant Quarantine , Chinese Academy of Inspection and Quarantine ,Beijing 100029;2.University of Kentucky)
Keywords:
Bean pod mottle virus ; PCR; ELISA
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2005.04.0317
Abstract:
Bean pod mottle virus is an economically important virus that infect soybean. A pair of specificprimers was designed based on the conserved region of the coat protein gene of bean pod mottle virus. To-tal RNA was extracted from infected soybean leaves and was reverse transcribed to cDNA. The cDNA wassubjected to PCR using the forward and reverse primers. PCR products of 650 bp were generated from allisolates tested. The RT - PCR method could be used to detect BPMV - infected soybean leaves with satis-fied results that were confirmed by DAS - ELISA assay. We recommended ELISA assay to detect BPMV -infected soybean seed rather than RT - PCR .

References:

1　 Gu, H. , Clark, A. J. , de Sa , P. B. , et al. Diversity among iso-lates of Bean pod mottle virus[ J] . Phytopathology , 2002, 92:446- 452.

2　 Giesler, L. J. ,Ghabrial S. A. , Hunt, T. E. , et al. Bean pod mot-tle virus a threat to U. S. soybean production[ J] . Plant Disease ,2002, 86:1280- 1289.
3　 Michelutti R ;Tu JC;Hunt DWA , et al . Anderson First report ofbean pod mottle virus in soybean in Canada[ J] . Plant - Disease ,2002, 86(3):330.
4　 Anjos JRN ;Brioso PST ;Charchar MJA. Partial characterizationof bean pod mottle virus in soybeans in Brazil[ J] . Fitopatologia -Brasileira, 1999, 24(1):85- 87.
5　 Fribourg CE;Perez W . Bean pod mottle virus (BPMV) affect-ing Glycine max (L.) Merr. in the Peruvian jungle[ J] . Fitopato-logia, 1994, 29(3):207- 210
6　 Zettler FW;Stansly PA ;Elliott - M S;et al. Repo rt of bean podmottle virus in South America[ J] . Plant - Disease, 1989, 73(6):518.

Memo

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Last Update: 2017-01-13