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AGROBACTERIUM - MEDIATED TRANSFORATION OF SOYBEAN WITH THE EXPRESSIONVECTOR CARRYING TWO SEPARATE T- DNAs(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2005年04期
Page:
291-295
Research Field:
Publishing date:

Info

Title:
AGROBACTERIUM - MEDIATED TRANSFORATION OF SOYBEAN WITH THE EXPRESSIONVECTOR CARRYING TWO SEPARATE T- DNAs
Author(s):
Zhang Xiuchun 1 　Gou Liqiong 1; 2 *Wu Kunxin 1 　Lin Junyang 3Lin Junfang 1; 2
(1. State Key Laboratory of Tropical Crop Biotechnology , Chinese Academy of TropicalAgricultural Sciences. Haikou 571101;2.Department of Bioengineering , College of FoodSciences,South China Agricultural University . Guangzhou 510640;3. Putian City’sInstitute of Agricultural Sciences. Putian,351100)
Keywords:
Co - transformation expression vector; Δ6- desaturase gene ; Transgenic soybean
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2005.04.0291
Abstract:
The co - transformation expression vector , pLIN61 carrying two separate T - DNAs with select-able marker gene and Δ6- desaturase gene was used to transformed soybean cotyledonary nodes mediatedby Agrobacterium tumef aciens strain EHA101. A batch of transgenic soybeans harboring Δ6- desaturasegene were obtained through Glufosinate screening. Research results demonstrate that the transformationefficiency , co - transformation frequency and real transformation frequency wrer 2. 0%, 56. 25% and1. 12 %respectively. PCR detection and Southern analysis confirm that borage Δ6- desaturase gene Gas in-tegrated into soybean genome. RT - PCR detection showed that borage Δ6- desaturase gene had expressedat transcriptional level in the transgenic soybeans.

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Last Update: 2017-01-13