|Table of Contents|

EFFECTS OF EXOGENOUS JASMONIC ACID ON THE ACCUMULATIONOF GLYCEOLLINS AND GLYCINOLLN GLYCINE MAX [ L.] MERRI.(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2005年04期
Page:
269-274
Research Field:
Publishing date:

Info

Title:
EFFECTS OF EXOGENOUS JASMONIC ACID ON THE ACCUMULATIONOF GLYCEOLLINS AND GLYCINOLLN GLYCINE MAX [ L.] MERRI.
Author(s):
Qi Yongyan 1Arnaud Bovy 2Ric de Vos 2Tang Yixiong 3
(1.Graduate School of Chinese Academy of Agricultural Sciences, Beijing , 100081;2.Plant Research International , P. O. Box 16 , 6700 AA , Wageningen , the Netherlands ;3. Biotechnology Institute of Chinese Academy of Agricultural Sciences , Beijing , 100081)
Keywords:
Glycine max [ L. ] Merri ; Cotyledons; Jasmonic acid; Glyceollins; Glycinol; High press liq-uid chromatography (HPLC)
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2005.04.0269
Abstract:
Health cotyledons were detached from 8 - day - old soybean seedlings (Zhonghuang No. 13). Asection of tissue , approximately 2×2mm was cut from the concave surface. 50μl jasmonic acid or silver ni-trate solution was dripped into the wound and incubation in the dark at 25℃. Glyceollins and glycinol wereextracted by methanol and analyzed by HPLC. The results show that there is no accumulation of glyceol-lins and glycinol in health cotyledons. Silver nitrate acts much stronger than jasmonic acid to induce the ac-cumulation of glyceollins and glycinol. The accumulation of glyceollins is in inverse proportion to the con-centration of jasmonic acid. The percent of 3 glyceollin isomer is 50 %～ 80%, 10%～ 40%and 10%～ 20%for glyceollin I, II and III, respectively. During the incubation, the ratio of glyceollin I decreases and thatof glyceollin II increases. It can be obviously observed in samples treated by silver nitrate.

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Last Update: 2017-01-10