THE RESEARCH ON TRANSFERINGCPT GENE INTO SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Author(s):: Wang Guiling 　 Huang Yongfen; (Harbin Normal University , Harbin 150080)

Abstract:: Cold resistance in the low temperature - induced plants is closely related to the synthesis metab-olism path of phospholipids. Cholinephospho transferase gene is a key enzyme gene in the synthesis metab-olism path of phospholipids. Phosphotidy choline(PC)is the enzyme gene product of CPT gene and it is thekey component of the membrane. . It is closely related with the mobility of membrane and cold resistance.In this experiment , plasmid PRDH401 which carrying the 35S - 35S - AMV - cpt - NOST fusiongene , was transferred into 5 varieties of soybean by means of the pollen tube pathwa. We got the D 1 genera-tion plants in the next year. Through the selection of kanamycin and low temperature, several transformedseedlings were obtained. PCR of the transformed soybean DNA indicated the integration of cpt gene intothe transgenic soybean genome.

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Last Update: 2017-01-08