|Table of Contents|

IDENTIFICATION OF THE TRANSGENIC ELEMENTS IN THE INTRODUCEDAMERICA SOYBEAN LINES(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2005年02期
Page:
116-120
Research Field:
Publishing date:

Info

Title:
IDENTIFICATION OF THE TRANSGENIC ELEMENTS IN THE INTRODUCEDAMERICA SOYBEAN LINES
Author(s):
Song Yang   Wu Cunxiang   Hou Wensheng   Han Tianfu
(Institute of Crop Sciences , The Chinese Academy of Agricultural Sciences, Beijing 100081)
Keywords:
T ransgenic soybean Glyphosate PCRCP4 - EPSPS
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2005.02.0116
Abstract:
In the present study , both field screening and PCR detection were conducted to identify thetransgenic elements in 46 accessions of introduced America soybean varieties. First , the introduced varie-ties were screened by foliar spaying of glyphosate at lethal concentration in the field;Secondly , CaMV35Spromoter , NOS terminator and lectin genes were examined by using PCR to determine if these lines weregenetically modified;Finally , CP4- EPSPS and lectin genes were amplified with PCR to determine if theywere glyphosate - resistant transgenic soybeans. The lectin gene of soybean itself was used as internalstandard to exclude the interference of DNA quality and experimental operation problems. Among the for-ty - six introduced America soybean varieties, five were found to be glyphosate - resistant and transgenic,and other forty - one were nontransgenic. The latter can be used as parents in soybean breeding program indifference regions of China.

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Last Update: 2017-01-08