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Mechanism of Sucrose Fatty Acid Ester PromotingInvertase Biosynthesis in Soybean Leaves*(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2005年02期
Page:
95-100，102
Research Field:
Publishing date:

Info

Title:
Mechanism of Sucrose Fatty Acid Ester PromotingInvertase Biosynthesis in Soybean Leaves*
Author(s):
Ye Xiaoli1 *　 Li Xuegang2
(1.School of Life Science , Southwest Normal University , Chongqing , 400715 ;2.Central Laboratory , Southwest Agricultural University , Chongqing , 400716)
Keywords:
Soybean ; Invertase ; SFE ; AMD; CHI
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2005.02.0095
Abstract:
Results showed that 2mmol /L of sucrose fatty acid ester (SFE) could increase the ac-tivity of invertase in soybean leaves, but no effects on the permeability of cell membrane includingthe leakage of K+and reducing glucose. The analysis of scanning on SDS - PAGE gels showedthat the increase of invertase activity after treated with 2mmol /L of SFE was caused by SFE en-hancing the content of increase. Experiments also indicated that 8mg /L of actinomycin D (AMD,a transcription inhibitor)and 1. 5mg /L of cycloheximede (CHI , a translation inhibitor)could de-cline the activity of invertase promoted by SFE in very short period obviously. It was suggestedthat SFE could increase invertase de novo biosynthesis on the level of the transcription mainly.

References:

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Last Update: 2016-12-23