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CONSTRUCTION OF TWO PLANT EXPRESSION VECTORS WITH SENSE ORANTISENSE SOYBEAN RESISTANCE- RELATED GENE SR1(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2005年02期
Page:
90-94
Research Field:
Publishing date:

Info

Title:
CONSTRUCTION OF TWO PLANT EXPRESSION VECTORS WITH SENSE ORANTISENSE SOYBEAN RESISTANCE- RELATED GENE SR1
Author(s):
Lin Shifeng 　 Zhang Shuzhen 　 Yang Xiuhong 　 Chen Qingshan 　 Yang Qingkai 　 Li Wenbin
(Institute of Soybean Science , Northeast Agricultural University , Harbin 150030)
Keywords:
Soybean SR1 gene ; Expression vectors; Transgenic ; Tobacco
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2005.02.0090
Abstract:
Two plant expression vectors carrying sense or antisense soybean resistance - related gene SR1under the regulation of cauliflower mosaic virus 35s promoter was constructed. Leaf segments of tobaccoHavana 425 were infected by Agrobacterium tumefaciens LBA4404 with pBISR1(+)or pBISR1(-), fromwhich kanamycin resistant plants were obtained. PCR and PCR - Southern analysis proved that the SR1gene was integrated into the genomes of the tobacco plants, and RT - PCR analysis proved that sense orantisense gene was transcripted into a complete mRNA. The disease resistance assay showed that plantswith antisense gene and control plants were slightly susceptible , and plants with sense gene were not sus-ceptible.

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Last Update: 2016-12-23