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FACTORS RELAXING THE SUBSTRATE INHIBITION IN LIPOXYGENASE- CATALYZINGHYDROPEROXIDATION OF LINOLEIC ACID(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2005年01期
Page:
52-55
Research Field:
Publishing date:

Info

Title:
FACTORS RELAXING THE SUBSTRATE INHIBITION IN LIPOXYGENASE- CATALYZINGHYDROPEROXIDATION OF LINOLEIC ACID
Author(s):
Su Yafen1　Zhou Jiangdong2　Fang Yun*1Xu Hongli1
(1. School of Chemical and Material Engineering , Southern Yangtze University , Wuxi 214036;2. Hangzhou Factory of Oil Chemicals , Hangzhou 311200)
Keywords:
Soybean - lipoxygenase ; Linoleic acid ; Substrate inhibition ; Relaxation; Hydroperoxide
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2005.01.0052
Abstract:
T he relaxation of substrate inhibition from additives in aerobic catalysis of hydroperoxidation ofsoybean linoleic acid by soybean lipoxygenase was studied in this paper. The results show that the additionof DMF can not only overcome the higher viscosity resulting from higher substrate level , improve the sub-strate solubility and enhance the coexistence of substrate with enzyme , but increase the yield of hydroper-oxide (HPOD). The higher initial oxygen is found also favorable to relaxing the excess substrate inhibi-tion. Antioxidant can control the side reaction of carbon- carbon fission of the HPOD and free radical initi-ated polymerisation. Under the optimal conditions of pH 9, 10 ℃,[ E] =3. 4 ×105 U/mL , φ(DMF)=7%,15 mL(O 2 )/min, ρ(sodium citrate)=0. 005 g /L , and 125 g(substrate)/L , 0. 08 g(antioxidant)/L wasadded 15 mins after and up to 88. 62 %HPOD was obtained 90 mins later.

References:

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Last Update: 2016-12-23