|Table of Contents|

GENETIC TRANSFORMATION OF PHYTOENE SYNTHASE GENE(PSY )INTO SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2005年01期
Page:
30-33
Research Field:
Publishing date:

Info

Title:
GENETIC TRANSFORMATION OF PHYTOENE SYNTHASE GENE(PSY )INTO SOYBEAN
Author(s):
Gong Xuechen 1; 2 Ji Jing 1 　 Kang Yanhong 2 　 Wang Gang 1 　 Wu Ying 3 　 Wang Ping 3
(1. TianjinUniversity , Tianjin , 300072;2. Hebei North University , Zhangjiakou, 075131;3. Huaihai Institute of Technology , Marine School , Lianyungang 222005)
Keywords:
PSY gene; Soybean ; Cotyledonary node; The Genetic transformation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2005.01.0030
Abstract:
T aking cotyledonary nodes of three varieties of soybean as acceptor , PSY gene were transformedinto soybean via agrobacterium - mediated. Hygromycin resistant regeneration plants of soybean were ob-tained by cotyledonary nodes inducting , striking root cultivation and stem elongation cultivation and hy-gromycin screening in selective condition. PCR and PCR - Southern analysis from the transferred geneplants proved that PSY has been transferred into genome of soybean. The factors affecting genetic trans-formation were also investigated.

References:

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Last Update: 2016-12-23