A RAPD MARKER RELATED WITH YELLOW SEED COATOF SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2005年01期

Page:

17-20

Research Field:

Publishing date:

Info

Title:

A RAPD MARKER RELATED WITH YELLOW SEED COATOF SOYBEAN

Author(s):

Wang Hui 1 　Duan Yuxi 1 * 　Chen Lijie 1 　Qiu Lijuan 2 　Chang Ruzhen 2

(1. Nematology Lab, Shenyang Agricultural University , Shenyang 110161 ;2. Key Lab of CropGermplasm &Biotechnology , Ministry of Agriculture , Instituteof Crops, Chinese Academy ofAgriculture Science , Beijing 100081)

Keywords:

RAPD; Molecular marker ; Seed coat color

PACS:

-

DOI:

10.11861/j.issn.1000-9841.2005.01.0017

Abstract:

BSA method was employed to analyze genome DNA of 11 black coat seed soybean and 12 yellowcoat seed soybean by RAPD method. The bands were produced for 66 primers among 100 primers. Therewere polymorphism bands for 15 primers. In all products amplified, a special DNA fragment , S79500 wasclosely related with the yellow coat of soybeans. The primer S79500 became a RAPD marker for the yellowcoat of soybean. In another hand, the S79500 also was used to test the filial generation with yellow ,green, black and brown coat from Liaodou 10 ×PI437654. The results showed that the S79500 was verystable and repeatable. The RAPD marker S79500 was useful to assistant the selection for soybean hybridbreeding.

References:

1　Michelmore R W , Paran I, Kesseli R V. Identification of markerslinked to disease - resistance genes by using segregating analysis:A rapid method to detect markers in specific genomic regions byusing segregating populations[ J] . Proc Natl Acad Science USA ,1991, 88:9828 - 9832.

Memo

Memo:

-

Common functions