T he effect of variant culture medium on callus inducement and shoot differentiated was studiedwith first lamina , cotyledons, hypocotyls explants of Yingxian Xiaoheidou. The results indicated that BAand NAA had no advantages on tissue culture of Yingxian Xiaoheidou, but combinations of cytokinin andauxin had advantages on callusinducement and organ differentiation.Culture mediums of Ms+BA2mg /L +IAA0. 5mg /LMs+BA1. 5mg /L +IAA0. 5mg /L and Ms +KT2mg /L +NAA0. 2mg /L ,had different rate ofplant regeneration with 46 %,46%, 16%respectively , In the first and second culture medium had got multi-ple shoot 。The rates of callus inducement and plant regeneration of soybean hypocotyls were higher thanthat of lamina and cotyledons.