|Table of Contents|

OPTIMIZATION OF GENETIC TRANSFOMATION SYSTEM IN SOMATIC EMBRYOS OFSOYBEAN MEDIATED BY AGROB ACTER IUM TUMEFACIENS(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2004年03期
Page:
200-204
Research Field:
Publishing date:

Info

Title:
OPTIMIZATION OF GENETIC TRANSFOMATION SYSTEM IN SOMATIC EMBRYOS OFSOYBEAN MEDIATED BY AGROB ACTER IUM TUMEFACIENS
Author(s):
Wang Xiaochun1;  2;  3Liu Shangqian3Ji Jing1 ;  2Wang Gang1 ;  2Wang Ping2
1.Tianjin Universi ty of Agricultural and Biological Engineering , Tian jin ;
2 .Changchun University of Agricultural and Animal Sciences , Changchun 130062 ;
3 .Department of Agronomy , HeBei North University , Xuanhua 075131
Keywords:
Soybean ; Somatic embryos ; Genetic transformation
PACS:
S 565.103.53
DOI:
10.11861/j.issn.1000-9841.2004.03.0200
Abstract:
The factors influencing genetic transformation about somatic embryos of soy bean had been studied .Mediated by Agrobacterium tumefaciens .The sui table condi tio n w as determined as followed :injured treatmentof somatic embryos of soybean , the bacterial concent ration reached 0 .5 -0 .7 (OD600) , 10 minutes infect iontime , 50 -100μmol/L AS .The t ransformation f requency increased in those condi tio n .

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Last Update: 2017-01-01