|Table of Contents|

cDNA CLONING AND PLANT EXPRESSION VECTOR CONSTRUCTIONOF SOYBEAN 11S GLYCUININ Gy5(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2004年03期
Page:
159-163
Research Field:
Publishing date:

Info

Title:
cDNA CLONING AND PLANT EXPRESSION VECTOR CONSTRUCTIONOF SOYBEAN 11S GLYCUININ Gy5
Author(s):
Wang Peiw u ;  Liu Lingzhi ;  Chai Xiaojie ;  Zhang Jun ;  Qu Tongbao
Biotechnology Center of Ji lin Agricultural University , Changchun 130118)
Keywords:
Soybean ; Gy 5 ; cDNA ; Clone ; Plant expression vector
PACS:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2004.03.0159
Abstract:
The total RNA w as est racted f rom immature seeds of soybean and direct ly used in single -stranded(ss)DNA synthetic reaction .According to soybean 11S g ly cinin Gy5 cDNA sequences in genebank , a pair ofprimers were designed .Double -stranded(ds)cDNA w as amplified by PCR 。The amplif ied Gy5 cDNA wascloned into plasmid pUC 19 and sequenced 。Fragment of the Gy5 cDNA in the recombinant plasmid w as cutw ith Pst I/EcoRI and ligated wi th plasmid Pcambia1301 , then a plant expression vector w as const ructed 。It wasalso t ransferred into Agrobacterium LBA4404 in o rder to be utilized later.

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Last Update: 2017-01-01