|Table of Contents|

DETERMINATION OF SOYBEAN ISOFLAVONE WITHULTRA-VIOLET SPECTROPHOTOMETRY(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2004年02期
Page:
147-150
Research Field:
Publishing date:

Info

Title:
DETERMINATION OF SOYBEAN ISOFLAVONE WITHULTRA-VIOLET SPECTROPHOTOMETRY
Author(s):
Yuan Jinbin1 　Lu Jianzhong2
1 .Pharmacy Research Cent re for TCM , Jiangxi Universi ty of Tradi tionalChinese Medicine , Nanchang 330006 ;
2 .Technology Department , J iangzhongPharmacy Group , Nanchang 330077
Keywords:
Soybean isoflavone ; UV spect rophotometry ; Determination
PACS:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2004.02.0147
Abstract:
A UV spect rphotomet ry used fo r determination of soybean isoflavone w as established .Genistein w asselected as the reference material , and the samples were determined at 325 nm .Influencing factors were dicussedand optimized .The total content of soybean isof lavo nes in soybean ex tracts is 38 .1 %w ith mean recovery102 .3 %, and the result for the function food is 4 .76 %w ith mean recovery 99 .9 %.This method is suitable forthe routine analysis and quality control of the funct ion food wi th easy -to -handle , good accuracy and reproducibility.

References:

1 　张立.RP-HPLC 法测定大豆提取物中大豆苷元、染料木素、大豆苷、染料木苷的含量[ J] .中草药, 2001 , 32(1):118 -120.

2 　赵世萍, 章育中.异黄酮含量的薄层光密度法测定[ J] .药学学报, 1985 , 20(3):203 -208.
3 　G .S .Mcleod , M .J .S heperd .Determination of the ionization constant s of isof lavones by capillary elect rophoresis[ J] .Czech .J .FoodS ci., 1999 , 17(2):61 -67 .
4 　G .J .Wang, O .Lapcik , R.Hampl , et al.Time-resolved f luoroimmunoassayof plasma daidzein and genistein[ J] .St eroids , 2000 , 65(6):339 -348.
5 　张玉梅, 孙学斌, 高旭年, 等.紫外分光光度法测定大豆总异黄酮的含量[ J] .中国食品卫生杂志, 2000 , 12(4):7 -8.
6 　鞠兴荣, 袁建, 汪海峰.三波长紫外分光光度法测定大豆异黄酮含量的研究[ J] .食品科学, 2001 , 22(5):46 -48.

Memo

Memo:
-
Last Update: 2016-12-31