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THE IDENTIFI ATION TECHNIQUEOF SOYBEAN LIPOXYGENASE(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2004年02期
Page:
111-113
Research Field:
Publishing date:

Info

Title:
THE IDENTIFI ATION TECHNIQUEOF SOYBEAN LIPOXYGENASE
Author(s):
Fu Cuizhen
Inst itute of Crop Germplasm Resources , CA AS , Beij ing 100081
Keywords:
Soybean ; Lipoxygenase ; IEF -PAGE
PACS:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2004.02.0111
Abstract:
The grass -bean flavor of soybean food stuff is related to lipoxygenase(Lox ).In processing , thephy sical or chemical methods are used to remove the undesirable flavo r and odo r of soy bean food stuf f , but thesemethods of ten lead to some insolubility of soybean proteins , and decrease the quality of protein .So it is necessary and urgent to select and develop mutant soybean by genetic study .Lipoxygenase lackingmutants (t riple - null o r double -null )soybean could improve nut ritional quality and functionality of soybeanproducts .There are four know n electrophoresis types of soybean seed lipoxy genase (Lox ) .The Lox isozymes havetheir ow n biochemist ry characteristic .IEF -PAGE helps us to identi ty Lox isozyme type and mutants .Wemodif ied the techniques about making gel and staining the gel fo r improving the resolution and reducing the experimentcost .The GelBond PAG sheet was replaced by no rmal glass plate .Use suitable enzyme staining solution.The tests attained remarkably result s and reduced cost .Thus , a technique fo r ident ifying Lox lackingmutants w as developed , that w as rapid and more accuracy .

References:

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2 　傅翠真, 徐文英, 苏震.中国大豆脂肪氧化酶缺失种质多样性鉴定研究[ J].中国农业科学, 1997 , 30(1):44 -51.
3 　Hildebrand D F , Versluys R T , Collins G B.Changes in lipoxygenaseisozyme levels during soybean embrgo development [ J] .Plant Science.1991 , 75 :1-8
4　Wang Wanheng , Tet suo Takano , Daisuke shibat a, et al.Molecularbasi s of a null mut at ion in soybean lipoxygenae 2 :S ubst itut ion of glutamine f or an i ron -ligand hi stidine [ J] .Proc.Nat l.Acad .Sci .USA., 1994 , 91 :5828 -5832 .
5 　Wang Wanheng , Tomohiko Kato , Tet suo Takano et al.Two single-base substi tutions involved in alt ering in a paired -box of AAATACin the promot er region of soybean lipoxygenase L -3 gene im pairthe promot er function in t obacco cells [ J] .Plant Science .1995 ,109 :67 -73 .

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Last Update: 2016-12-31