|Table of Contents|

CHARACTENRIZATION OF RESISTANCE GENES TO COTTON WORM WITH SSRMARKERS IN SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2004年02期
Page:
91-95
Research Field:
Publishing date:

Info

Title:
CHARACTENRIZATION OF RESISTANCE GENES TO COTTON WORM WITH SSRMARKERS IN SOYBEAN
Author(s):
Wang Hui　Yu Deyue 　Wu Qiaojuan 　Gai Junyi
Soybean Research Institute , Nanjing Agricultural University , National Center for Soybean Improvement ;National Key Laboratory of Crop Genetics and Germplasm Enhancement , Nanjing , 210095
Keywords:
Soybean(Glycine max (L .)Merrill); Cot ton Wo rm(Prodenia litura (L .)Fabricius ) ; Ant ibiosisto insect ; SSR
PACS:
S 565.103.4
DOI:
10.11861/j.issn.1000-9841.2004.02.0091
Abstract:
There have been few reports about molecular marker analy sis of resistance to insect in soybean .Thisstudy w as to determine quantitative trait loci (Q TLs)associated with the soybean resistance to insect (Co ttonWorm)by estimating linkage to simple sequence repeat (SSR)markers.Recombinant inbred lines (RILs)constructed through a cross betw een Wan82 -178 and Tong shanbopihuangdoujia w as used .Larval w eight w as examinedin as index of resistance .Using the interg rated genetic linkage map of soy bean (Cregan et al .1999), wechose 103 SS R markers scattering on 7 linkage g roups (LGs)that had the po tential to map resistant QTL toscreen polymorphic markers betw een Wan82 -178 and To ngshanbopihuangdoujia .It w as found that 18 SSRprimers show ed polymo rphic DNA bands among these RIL lines.Wi th t -test analy sis and analysis of variance ,three markers (Satt135 , Sat t363 , Sat t434)w ere found linked w ith the resistance genes , situat ing on three LGs,i .e .D2 , C2 , and H .The individual ef fect of each marker was reported as R2 value , i .e 5 .57 %, 2 .89 %, and8 .7 % respectively .

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Last Update: 2016-12-31