STUDYON QUALITATIVE TESTOFTRANSGENES BY PCR-ELISA IN SOYBEAN CULTIVARS(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2004年01期

Page:

55-58

Research Field:

Publishing date:

Info

Title:

STUDYON QUALITATIVE TESTOFTRANSGENES BY PCR-ELISA IN SOYBEAN CULTIVARS

Author(s):

Lei Bojun¹Lu Xiaobo¹Shan Hong¹Zhu Shuifang²Chen Hongy un²Zhao Wenjun²

1.Heilongj iang Academy of Agricultural Sciences , Harbin 150086 ;

2.Quarantine Institute in Animals and Plants of S tate Quality Test Bureau , Beijing 100029

Keywords:

Soybean ; T ransgene ; Di rect int roduction exogenous DNA ; Quali tative analy sis

PACS:

S 565.1

DOI:

10.11861/j.issn.1000-9841.2004.01.0055

Abstract:

The oliqonucletides w hich covalent primers , bonded on the w all of a PCR tube as solid primers , havebean amplified by PCR equipment .The Solid and Liquid phase amplified by PCR were hy bridized and done w ithgel -eleitro phoresis respectively .Some Heilongjiang soybean cultivars, Suchas Hefeng 35 and Heinong 37(bredby conventional breeding), Heisheng 101(bred by introducing exogenous DNA through pollen tube channel , andSoybean of American 2 from Dalian importing , were tested by the PCR -ELISA .The result showed that Soybeanof American 2 w as a transgenic soy bean , Heisheng 101 , Hefeng 35 and Heinong 37 w ere no t .Those haveimportant meaning in plant molecular breeding , ecological pro tecting and safety monito ring .

References:

1 　赵文军, 陈红运, 黄文胜.PCR -ELISA 在转基因产品检测中的应用[ J] .植物检疫, 2001 , 15(5):297-299.

Memo

Memo:

-

Common functions