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STUDY ON CRITICAL AND SCREEN SOYBEAN GROWOF KANAMYCIN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2003年04期
Page:
261-263
Research Field:
Publishing date:

Info

Title:
STUDY ON CRITICAL AND SCREEN SOYBEAN GROWOF KANAMYCIN
Author(s):
YuanYing 　Liu Depu 　Wang Yumin 　Zheng Peihe
Jilin ProvinceKey Open Labor On Agora -Biotechnology , Gongzhuling 136100
Keywords:
Soybean ; Kanamycin ; Screen ; Restrain
PACS:
S 565.101.2
DOI:
10.11861/j.issn.1000-9841.2003.04.0261
Abstract:
The effects of kanamycin on seed germination , growth of cotyledon , rooting of shoots were investigated using 3soybean varieties.The results showed that the effect of kanamycin on different explants varied significantly.The critiealconcentration was 1000ppm .When soybeans seeds were soaked with kanamycin solution where as the cribical concentrationwas 50 -100ppm ,when soybeans were inoculated on media added kanamycin .For cotyledon node , the certain concentrationof kanamycin was 100ppm .None of shoot tips and shoots regenerated from cotyledon cuture could root even inthe media with lowest concentration of kanamycin .The effect of kanamycin showed slight on different genotypes .Thescreening for critical concentration of kanamycin must be done before the screening of transformants .

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Last Update: 2016-12-31