THE EXPRESSION CHARACTER OF DEFENSE ENZYMES ANDSOLUBLE PROTEINS IN THE EARLY INTERACTION BETWEENSOYBEAN AND Cercospara soj ina(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Title:: THE EXPRESSION CHARACTER OF DEFENSE ENZYMES ANDSOLUBLE PROTEINS IN THE EARLY INTERACTION BETWEENSOYBEAN AND Cercospara soj ina

Author(s):: Gao Xuew en¹Yang Chun¹Fan Jiankun¹Ding Junjie²Zheng Tianqi²Ma Shumei²; 1 .Key Laboratory of Moni toring and Management of Plant Diseases and Insects , Ministry ofAgriculture , Nanjing Agricultural University , Nanjing 210095 ;
2 .Inst itute of Hejiang Agricul tralScience Research , Hei longjiang Academy of Agricul tral Sciences , Jiamusi 154007

Keywords:: Soybean ; Cercospora sojina ; Interaction ; Defense enzymes ; Isoenzyme ; Soluble protein

Abstract:: Expression character of defense enzymes and soluble proteins in the interaction betw een soybean sing lev ariety and Cercospora sojina dif ferent strains or dif ferent varieties and single st rain w ere assayed .Result sshowed that POD and SOD activity in both incompatible or compatible interaction were increased , but the increaseof POD and SOD activity in incompat ible interaction was higher and faster than that in compatible interaction, w hile the number of isoenzyme bands w as not changed in both interaction , that implied the increase ofPOD and SOD activity w as related w ith increase of isoenzymes act ivity .Electrophoresis show ed that some solubleproteins w ere increased in both incompatible and compatible interaction , they are pathogenesis -related proteins(PR protein).The fast and marked expression of POD activity in the soybean varieties w hich w as inoculatedw ith sing le st rain is also related w ith incompat ibility of soybean and Cercospara soj ina .The results indicatethat fast and marked increase of defence enzymes POD and SOD activity is a character of incompatible interact ionbetween soybean and Cercospara sojina .

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Last Update: 2016-12-31