|Table of Contents|

TRANSFORMATION OF SOYBEANWITH FLORAL REGULATORY GENE GAGA1(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2003年02期
Page:
79-82
Research Field:
Publishing date:

Info

Title:
TRANSFORMATION OF SOYBEANWITH FLORAL REGULATORY GENE GAGA1
Author(s):
Yu Deyue1Wei Guolan1Sun Ying1Teemu H Teeri2
Nanjing Agricultural University , Soybean Research Insti tute , National Center for SoybeanImprovement , National Key Laboratory of Crop Genetics and Germplasm Enhancement ,Nanjing 210095 , Inst itute of Biotechnology , 00014 -University of Helsink i ,Finland
Keywords:
Regulato ry genes ; Genetic transformat ion ; Pollen tube pathw ay ; Soybean
PACS:
S 565.103.53
DOI:
10.11861/j.issn.1000-9841.2003.02.0079
Abstract:
Transformation of soybean w ith gaga1 was conducted using several accessions includingN2899 ,Tongzhoudou , 95 -7etc .through pollen tube pathw ay .About 3000 seeds w ere obtained from 4300 flowerstreated w ith Ti plasmid pHT T613 .By indirect identification method w ith Kanamycin in the field , 14 plant s obtainedw ere found resistant to Kanamy cin .These 14 plants were examined by PCR w ith NPT Ⅱ and gaga 1 specificprimers .Tw o N2899 plant show ed positive signals .This indicated preliminarily that gaga1 w as integratedinto the genome of the tw o plants .These tw o t ransgenic plants were shorter and had less flowers during fullblooming period , but no obvious modification was found in f lo ral st ructure compared to unt ransformed N2899 .The detailed characterization of t ransgenic plants shall be carried out in further generations .

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Last Update: 2016-12-31