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EFFECTION OF PROLINE AND AgNO3 ON AGROBACTERIUM-MEDIATEDTRANSFORMATION OF SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2003年01期
Page:
36-39
Research Field:
Publishing date:

Info

Title:
EFFECTION OF PROLINE AND AgNO3 ON AGROBACTERIUM-MEDIATEDTRANSFORMATION OF SOYBEAN
Author(s):
Liu Jinhua1Wang Peiwu2Wu Limin2Yu Yanchun2Zhan Jun2Hao Wenyuan2Yao Dan2
1 .Center of Technology , Jilin Entry -Exit Inspection and Quarantine Bureau of the P .R .CChangchun 130062 ;
2 .Center of Biotechnology , Jilin AgriculturalUniversity , Changchun 130118)
Keywords:
Soybean ; Agrobacterium tumefaciens ; Genetic transformation ; Proline ; AgNO3
PACS:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2003.01.0036
Abstract:
Agrobacterium tumefaciens has been used extensively for genetic engineering in soybean , but to date , transformationfrequency of soybean is still low relative to that obtained in other plants .At an attempt to improve transformationefficiency , present experiment was conducted to add two compounds on transformation of cotyledonary nodes in soybeanvia Agrobacterium -mediation .The experiment result indicated that 2mg L proline or 2mg L AgNO3 had the optimal effect on soybean transformation, the GUS gene tansformation rate was increased obviously .

References:

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Last Update: 2016-12-31