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A PRELIMINARY STUDY ON TRANSFERRING ■6 -FATTY ACID DESATURASEGENE FROM MORTIERALLA ISABELLINA INTO SOYBEANS(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2003年01期
Page:
11-17
Research Field:
Publishing date:

Info

Title:
A PRELIMINARY STUDY ON TRANSFERRING ■6 -FATTY ACID DESATURASEGENE FROM MORTIERALLA ISABELLINA INTO SOYBEANS
Author(s):
Bu Yunping 　Li Mingchun 　Hu Guowu 　Wang Guangke 　Xing Laijun
Department of Microbiology , Nankai University , Tianjin 300071
Keywords:
Soybean ; ■6 -fatty acid desaturase gene ; Agrobacterium infection ; Transferred gene plant
PACS:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2003.01.0011
Abstract:
The ■6 -fatty acid desaturase gene fron Mortitieralla isabellina was transferred into cultivars Jiyu 43 ,Heinong36 , Heinong 37 , et al .via Agrobacterium infection by inducting with various explant methods .Adentitious budsand regeneration plant were inducted from cotylendon node , hypocotyl .Kanamycin resistant (Kmr)plantlets were obtainedby 3 -4 cycles screening in selective condition .PCR and Southern blot analysis from the transferred gene plantsproved that foreign gene were transferred and integrated into soybean genome .

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Last Update: 2016-12-31