|Table of Contents|

STUDY ON THE CHANGEOF POD ACTIVITY AND PPO ACTIVITYIN SOYBEAN VARIETIESWITH DIFFERENT RESISTANCE TO Pseudomonas syringae pv.Glycinea (PSG)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2002年03期
Page:
183-186
Research Field:
Publishing date:

Info

Title:
STUDY ON THE CHANGEOF POD ACTIVITY AND PPO ACTIVITYIN SOYBEAN VARIETIESWITH DIFFERENT RESISTANCE TO Pseudomonas syringae pv.Glycinea (PSG)
Author(s):
Zhou Boru1Liu Taiguo1Yang Wei2Li Yong hao1
1.Northeast Agricul tural University , Harbin 150030 ;
2.Popularization Centreof Agricultural Technique of Hei longj iang Province , Harbin 150030
Keywords:
Soybean ; Pseudomonas syringae pv .glycinea ; Resistance ; Peroxidase ; Polyphenoloxidase
PACS:
S565.133.22
DOI:
10.11861/j.issn.1000-9841.2002.03.0183
Abstract:
Two soy bean varieties, Suinong 8 and Dong nong 42 , susceptible to PSG , and tw o soy bean varieties,Heinong 37 and Heinong 38 , resistant to PSG , w ere grown in the g reenhouse .They were inoculated wi th PSGat the t ri -leaf stage .The uninoculated plant s w ere used as control.The first leaves were taken for biochemicalanaly sis from the second day after inoculation .The result s show ed that there was no difference in Peroxidase (POD)and Polyphenoloxidase (PPO)inhealthy soy bean .Between resistant and susceptible varieties , the POD act ivity in all 4 varieties increased af ter inoculationwi th PSG .But they were higher in resistant cultivars .The PPO activity increased in resistant cultivars, how ever it decreased in suscept ible ones .The number of PPO isoenzyme bands w as same in all cultivars.But POD isoenzyme bands (A2 and B2)were two more only in the resistant varieties than in the susceptible ones.The color of the POD C1 bands got deeper in resistant varieties after inoculation , w hile the B1 , B2 , C2 and B4bands go t faded .The color of the PPO bands of B1 and D1 faded and the C2 band g ot deeper after inoculation insusceptible v arieties .However , the colo r of the B1 band got deeper and C2 band faded in resistant variet ies af terinoculat ion .

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Last Update: 2016-12-30