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ESTABLISHMENTOF EMBRYOGENIC SUSPENSION WITH IMMATURE COTYLEDONSOF SOYBEAN (Glycine max (L.)Merr.)AND INDUCTION OF SECONDARY EMBRYOS(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2002年02期
Page:
123-126
Research Field:
Publishing date:

Info

Title:
ESTABLISHMENTOF EMBRYOGENIC SUSPENSION WITH IMMATURE COTYLEDONSOF SOYBEAN (Glycine max (L.)Merr.)AND INDUCTION OF SECONDARY EMBRYOS
Author(s):
Li Haiy an 　Zhu Yanming 　Feng Yingying 　Zhou Changmei 　Liu BeidongDing Xiaodng 　Zhang Shuzhen
Department op Plant Bioengineering , College of Life Sciences NortheastAgricultural University , Harbin 150030
Keywords:
Soybean ; Immature coty ledons ; Embryogenic suspension system ; Secondary embryos
PACS:
S 565.1
DOI:
10.11861/j.issn.1000-9841.2002.02.0123
Abstract:
With 5 soybean cultivars in Heilong jiang province , somatic embryos were induced wi th immaturecotyledo ns and secondary embryogenesis systems were established through suspension proliferation ofembryogenic cultures .We studied systematically the influence of component and co ncent ration of medium onsomatic embryo induction and suspension culture proliferatio n .The result s show , high efficient medium ofinducing somat ic embryos is MSO +40mg/L 2 , 4 -D +6 %sucrose .High eff icient medium of global embryosproliferation is 10mg/L 2 , 4 -D +1/2 MS nit rogen +5mM glutamine +5mM asparagines .The percentage ofembryos mature and regeneration of secondary embryos af ter cultured for 8 weeks were sig nificantly improved ,and reached 63 % and 35 % respectively at the 12 week , af ter then , the increased rate declined .

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Last Update: 2016-12-30