STUDYON FACTORS AFFECTING THE SOYBEAN COTYLEDONARY NODE REGENERATION(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2002年02期

Page:

88-92

Research Field:

Publishing date:

Info

Title:

STUDYON FACTORS AFFECTING THE SOYBEAN COTYLEDONARY NODE REGENERATION

Author(s):

Liu Beidong ¹ZhuYanming²Li Haiy an²Yang Qian¹

1.Department of Li fe Science and Engineering , Harbin Inst itute of Technology , Harbin 150001 ;

2.Li fe Science Col lage , Northeast Agriculture University , Harbin 150030)

Keywords:

Soybean ; Coty ledonary node ; Multiple shoot ; Sonication assisted t reatment

PACS:

S 565.035.3

DOI:

10.11861/j.issn.1000-9841.2002.02.0088

Abstract:

To establish a high frequency regeneration system for the gene t ransfo rmation of soybean(Glycinemax (L .)Merr .), cotyledonary nodes f rom ten varieties w ere used as the ex plants in the regenerat ionexperiments.Six factors such as geno types , plant grow th regulators , sonication assisted t reatment and so onw ere studied .The result s show that the high regeneration f requency geno types are Hefeng 35 , Hefeng 25 ,Heinong 40 , the multiple shoot s per explant are 6 .69 , 6 .32 and 5 .98 respect ively .The BA concentrationsrequired in shoot dif ferentiation medium are different among these geno types, in Hefeng 35 , 1 .5mg/l Hefeng 25and in 1 .2mg/l Heinong 40 .But the IBA concentrations in root regenerat ion medium are the same .The fullcotyledo nary is the best explant size for shoo t differentiation .As an antibiotic to restrain the propagatio n ofexcess Ag robacterium , the Cef has no sig nificant inf luence on regeneration .When the t reatment time is shorterthan 12s , the sonicat ion assisted t reatment has no sig nificant influence on the regeneration of shoots .

References:

1 　邓向阳, 卫志明.大豆转化技术[ J] .植物生理学通迅, 1998 , 34(5):381 -387.

Memo

Memo:

-

Common functions