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CYTOLOGICAL STUDIES ON POLLEN ABORTION INCYEOPLASMIC-NUCLEARMALE STERILE SOYBEAN LINS NJCMS1A(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2001年03期
Page:
167-171
Research Field:
Publishing date:

Info

Title:
CYTOLOGICAL STUDIES ON POLLEN ABORTION INCYEOPLASMIC-NUCLEARMALE STERILE SOYBEAN LINS NJCMS1A
Author(s):
Ding Derong 　Gai Juny i
Soybean Research Insti tute , Nanj ing Agricul tural University ;National Center ofSoybean Improvement , Minist ry of Agriculture , Nanjing 210095
Keywords:
Soybean ; Cy toplasmic -nuclear male sterility ; Microspore development ; Cy tochrome oxidase
PACS:
S565.101
DOI:
10.11861/j.issn.1000-9841.2001.03.0167
Abstract:
Comparative observation on microspore development in cy toplasmic -nuclear male sterile soybean material, such as (N8855 ×N2899)F1 , BC1 male sterile plant s , NJCMS1A and i ts fertile countpart (N2899 ×N8855)F1 , maintainer line , w as made w ith light and transmission electron microscopy .The result show ed theabortion appearance of the dif ferent generatio ns with the same cy toplasm w as similar , pollen abo rtion occurredmajorly f rom late mononucleate stage to binucleate stage .The abno rmal vacuoles in microspo re in (N8855 ×N2899)F1 male sterile anther occurred at late mononucleate microspore stage .Plasmodium shriveled at maturepollen g rain , The partial cy toplasm disinterg rated , and 2 -3 nucleus w ere found in some microspores in BC1sterile plants .Pollen abortion of NJCMS1A occurred at the stage of binucleate pollen .There appeared mo resmall vacuoles in microspo re cy toplasm of NJCMS1A than that of it s maintainer line at the stage that generativecell closed to vegetative cell , starch grains not being produced in cytoplasm .After generative cell w as aw ay f romvegetative cell , part of cytoplasm disordered , generative and vegetative nuclei further degenerated , vacuoles expanded, part of cy toplasm disinteg rated , and the development of endexine w as completely inhibited .Isozymeanaly sis indicated that the fastest cytochrome oxidase active band missed at binucleate stage , w hich w as consistentw ith the result of the pollen abortion phase .Thus , the mechanism of the cytoplasmic -nuclear male sterilityw as probably associated w ith cy tochrome o xidase .

References:

1 　戴尧仁, 孙振荣, 徐月荣, 等.水稻二九南一号雄性不育系及相应保持系花药中某些呼吸酶和游离组蛋白的比较研究[ J] .遗传学报, 1978 , 5(3):227 ～ 233.

2 　丁德荣, 盖钧镒, 崔章林, 等.大豆质核互作雄性不育系NJCMS1A 及其保持系NJCMS 1B 的选育与验证[ J] .科学通报,43(17):1901 ～ 1902.
3 　孙寰, 赵丽梅, 黄梅.大豆质核互作不育系研究[ J] .科学通报,1994 , 38(16):1535 ～ 1536.
4 　武汉大学遗传研究室, 红芒野稻-莲塘早不育系花粉败育过程的细胞形态学观察[ J] .植物学报, 1978 , 20(2):150 -155.
5 　许占友, 李磊, 邱丽娟, 等.大豆三系的选育及恢复基因的SSR初步定位研究[ J] .中国农业科学, 1999 , 32(2):32～ 38.
6 　杨守萍, 盖钧镒, 徐汉卿.大豆雄性不育突变体NJ89 -1 的遗传与细胞学研究[ J] .大豆科学, 1998 , 17(1):32 ～ 38.
7 　张磊, 戴瓯和.大豆质核互作雄性不育系W931A 的选育[ J] .中国农业科学, 1997 , 30(6):90 ～ 91.
8 　Davis , W.H .Rout e t o hyb rid soybean production[ J] .U .S .Patent.1985 , 4 545 146.
9 　Gai Junyi , Cui zhanglin , Ji Dongfeng , et al.A report on the nuclearcytoplasmic male steri li ty f rom a cross betw een two soybean cult ivars[ J] .S oybean Genet ics Newslett er , 1995, 22 :55 ～ 58.
10 　Jin Wei , Horner H T , Parlmar R G .Genet ics and cy tology of anew geni c male-st erile soybean[ Glycine ma x (L)Merr] [ J] .SexualPlant Reproduction .1997 , 10(1):13 ～ 21.
11 　Laser, K .D.N .R.Lerst en.Anatomy and cyt ology of microporogenesisin cy toplasmi c male sterile angi osperms[ J] .Bot.Rev .,1972 , 38(3):425 ～ 454.
12 　Robert A .Grayboach , Palmer, R.G ., Male steri li ty in soybean :an overview[ J] .American J .Bot .1988 , 75(1):144 ～ 156.
13 　Watson , C .V ., J .Possible mit ochondrial involvement in mechanism of cytoplasmic male steri li ty in m aize (Zea mays L.)[ J] .Nathand Dave Nanda.Biochem Genet ics , 1977 , 15 :1113 ～ 1124.

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