INTRODUCTION OF Bt GENE(cryIA)INTO SOYBEANBY Agrobacterium -MEDIATED TRANSFORMTION(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2001年03期

Page:

157-162

Research Field:

Publishing date:

Info

Title:

INTRODUCTION OF Bt GENE(cryIA)INTO SOYBEANBY Agrobacterium -MEDIATED TRANSFORMTION

Author(s):

Zhou Sijun¹Li Xichen¹Liu Zhaojun¹Liu Liyan¹Yang Qingkai²

1.Biotechnology Research Center , Heilongjiang Academy of Agricultural Sciences , Harbin 150086 ;

2.Insti tute of Soybean , Northeast Agricultural University , Harbin 150030

Keywords:

Soybean ; Transgenic plant ; Bt gene ; Transformation

PACS:

S565.035.3

DOI:

10.11861/j.issn.1000-9841.2001.03.0157

Abstract:

Baci llus thuringiensis cry IA gene w as int roduced into soybean successfully w ith Agrobacterium -cotyledo nary node t ransformation system .Cotyledonary node ex plants were prepared f rom 5 -7 day oldseedlings , infected and co -cultivated with Agrobacterium tumefaciens .Resistant adventitious buds emerged after4 weeks on selective medium .The buds w ere t ransferred onto elongation medium and regenerated shoot sg rew to 2 .5 -3 cm high af ter 4 -6 w eeks .The shoo ts were cut off and t ransferred onto root ing medium .Af terabout 3 weeks , the rooted plantlets w ere hardened of f and t ransferred into pots .All the plant s could f low er andset seeds normally .Among the 8 t ransplanted survival T0 plant s, seven gave posi tive PCR reaction ;and 4 of the7 T1 plant lines had PCR posi tive plant s .DNA w as ex tracted f rom the leaves of positive plants in the 4 T1 plantlines , and southern blo t w as conducted with probe of Bt gene .All the 4 lines are positive , indicating that the Btgene has integrated into the receiver’ s genome and can be passed to progenies .

References:

1 　周思军.大豆遗传转化的主要障碍及研究进展[ J] .黑龙江农业科学, 2001 ,(3):24 -27 .

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