|Table of Contents|

STUDY ON PLANT REGENERATION FROM SOYBEAN CULTURE(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2001年01期
Page:
9-13
Research Field:
Publishing date:

Info

Title:
STUDY ON PLANT REGENERATION FROM SOYBEAN CULTURE
Author(s):
Yuan Ying1Liu Depu1Zheng Peihe1Xu Wenjing1Li Hailo ng2
1. Jil in Province Key Open Labor On Agro- Biotechnology ;
2. J iLin Mechanizat ion in Agriculture School , Jil in Province
Keywords:
Soy bean;  Co tyledo n;  Multiple shoot;  Plant reg eneratio n
PACS:
S565. 035. 3
DOI:
10.11861/j.issn.1000-9841.2001.01.009
Abstract:
High f requency multiple shoo t- bud fo rmatio n f rom di fferent g eno type of soybea n co tyledonnode fo r the tw o- w eek pre- culture and two- week shoo t- inducing culture on a 1 /2MS- medium supplementedwi th BA and IBA w as o btained. Plant can be o btained f rom mul tiple sho ot- bud when t ra nsferredto low concentration o f BA, and af terw ards to f resh roo ting medium supplement N AA. 20- 30 budsw ere o btained f rom per ex pla nt fo r 40- 60 day s culture. It w as found that the plant reg eneratio n f requencyva ries obviously from g eno ty pes, hormo nes , dif ferent ex cision ci te a nd inducing time . Sui table conditionand procedure w ere established for the clump buds fo rmatio n and plant reg eneration, and this high f requencyreg eneratio n system ca n be applied to the soybean g enetic t ransfo rmatio n by ag o ra- bacterium a ndparticle bombardment.

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Last Update: 2016-12-28