|Table of Contents|

A STUDY ON AMPLIFIED FRAGMENT LENGTH POLYMORPHISM( AFLP) IN SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2000年03期
Page:
210-217
Research Field:
Publishing date:

Info

Title:
A STUDY ON AMPLIFIED FRAGMENT LENGTH POLYMORPHISM( AFLP) IN SOYBEAN
Author(s):
Tian Qing zhen1　Gai Junyi1?　Yu Deyue1　Jia Ji zeng2
1. Soybean Research Institute, Nanjing Agriculture University; National Center ofSoybean Improvement , Ministry of Agricul ture, Nanjing , 210095;?
2. Institute ofCrop Germplasm Resour ces , Chinese Academy of Agricul tural Science; KeyLaboratory of Crop Germplasm & Biotechnology , Ministr y of Agr iculture, Bei jing , 100081
Keywords:
Soybean;  DNA ex traction;  Silv er - s taining inspection;  AFLP ( amplifiedf ragment leng th polymo rphism)National Key Science and Technology Pro ject No. 960140106Author f or correspondence
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2000.03.0210
Abstract:
In the present paper, improvement of the DN A ex traction f rom dry soybean s eed,comparison between the silv er s taining and isot ope labeling methods, and ex plora tion of theendoenzyme and primer combinations sui table f or AFLP analysis in soybeans were presented.Based on this , a simple and f as t procedure suitable fo r soybean AFL P analysis w asestablished, and the repres entativ e accessions of G. soja and G.max f rom v arious ecologicalareas in China were examined by using the sugges ted procedure.

References:

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Last Update: 2016-12-27