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RAPD ANALYSIS OF THREE SOYBEAN LINES FROMTHE CROSS OF ( Glycine soja× Glycine max ) × G. max(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2000年02期
Page:
97-104
Research Field:
Publishing date:

Info

Title:
RAPD ANALYSIS OF THREE SOYBEAN LINES FROMTHE CROSS OF ( Glycine soja× Glycine max ) × G. max
Author(s):
Zheng Cuiming1　Chang Ruzhen1　Qiu Lijuan1　Li Yuqing2　Guo Pei1
1. Inst itute of Crop Germplasm Resources, CA AS , Bei jing 100081, China;
2. Institute of Agricul tural Science in Chengde City , Hebei Prov ince
Keywords:
Soy bean lines;  Glycine soja × Gl ycine max ;  RAPD markers
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2000.02.0097
Abstract:
Three soybea n eli te lines( 8650- 1- 4 a nd 8702- 2- 4, 8702- 2- 8) w asdev elo ped by using Gly cine soja as female parent and Glycine max as male parent. 8650- 1- 4 was dev elo ped by backcrossing o f To ng jiao 81- 1543 wi th F1 o f ( G. so ja ×Chengdou No. 1) , and 8702- 2- 4, 8702- 2- 8 w ere dev eloped by backcrossing ofTongjiao 81 - 1543 wi th F2 o f ( G. soja × Cheng do u No. 1) . Parental and prog enynuclear DN A w as analy sised by RAPD method. A total o f 79 primers were a naly zedw ith parent s, fi f teen of w hich w ere po lymo rphic betw een parents. Using these 15primers, G. Soja amplified 19 special loci, 4 in Cheng dou a nd 9 in To ng jiaorespectiv ely , which indica tes that genetic backg round of G. soja di ffer f rom G. max .The number of special loci coming from G. so ja , Chengdou No. 1 and Tong jiao 81- 1543is 2, 2 and 5 for 8702- 2- 4; . 1, 2 and 8 fo r 8702- 2- 8; 11, 1 and 2 fo r 8650- 1- 4,respectiv ely. These resul ts w ere in accorda nce wi th their ag ro nomic characterperfo rmance, 8702- 2- 8 w as mo re simila r to Tong jiao 81- 1543 and 8650- 1- 4inheri ted more characters f rom G. soja , w hich indicated tha t RAPD markers i s an usefultoo l to study rela tionship betw een pa rent s and prog enies . In addition , w e also foundsome RAPD loci w ere st ronger in 8702- 2- 4 a nd 8702- 2- 8 than thei r parents, butthe mechani sm i s no t clear.

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Last Update: 2016-12-27