|Table of Contents|

THE TRANSFORMATION OF FOREIGN GENEMEDIATED BY POLY- L- ORNITHINE(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2000年01期
Page:
26-30
Research Field:
Publishing date:

Info

Title:
THE TRANSFORMATION OF FOREIGN GENEMEDIATED BY POLY- L- ORNITHINE
Author(s):
Nan Xiang ri
Biotechnology Research Center , Heilongjiang Academy ofAgriculture Science, Harbin 150086
Keywords:
Poly- L- Orni thine;  Pro to plast;  Foreig n DN A
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2000.01.0026
Abstract:
Poly - L - Ornithine ca n change the electric charge o n the surface ofpro toplasts or fo reig n DN A. The pro toplasts and the foreing DN A which carry negativ eelectric charge o riginally become partially ca rring po sitiv e charge and enha nce theint roductio n of the fo reig n DN A into the pro to plast s. The resul t o n t ransforma tion ofsoybean v arieties, Heino ng 35 and Heino ng 37, with BT g ene mediated by PLO show edthat the method is feasible and the t ra nsfo rmatio n frequency w ent up to 0. 33% .

References:

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Last Update: 2016-12-27