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HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATIONOF ISOFLAVONE CONTENT IN SOYBEAN TEST SAMPLES(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2000年01期
Page:
15-20
Research Field:
Publishing date:

Info

Title:
HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATIONOF ISOFLAVONE CONTENT IN SOYBEAN TEST SAMPLES
Author(s):
Sun Junming1　Ding Anlin1　Do ng Hui ru2
1. Inst itute of Crop B reeding and Cult ivat ion , CAA S , Beij ing, 100081
2. Inst itute of Vegetables and Flowers, CA A S, Bei jing , 100081
Keywords:
Soy bean ( Glycine max ) ;  HPLC;  Isof lav one
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2000.01.015
Abstract:
Soy bean isoflav one compounds w ere separated and determined w ithHPLC, a nd a simple sensi tiv e and hig hly reproducible method wa s established. Theresul t indica ted the mo st suitable ex t racting time of i sof lav ine is 2- 3h r, the sui tableabso rbent w av eleng th is 260nm; It i s accurate for th e iso flavo ne standard sample w ithdaidzin and g enistin at the range of concent ra tion; The i sof lav o ne co ntent is af fected byex t racting and examining temperatures. At 80℃ or higher tempera ture, i sof lav o nemalonyl conjug ates are hydroly zed to glucosides and ag lycones.

References:

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Last Update: 2016-12-27