RAPID ISOLATION OF SOYBEAN DNA FOR PCRWITH LARGE NUMBERS OF SMALL SAMPLES(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

1999年04期

Page:

318-321

Research Field:

Publishing date:

Info

Title:

RAPID ISOLATION OF SOYBEAN DNA FOR PCRWITH LARGE NUMBERS OF SMALL SAMPLES

Author(s):

Zhou Si jun

Biotechnolog y Research Center, Heilongjiang Academyof Agricul tural Sciences, Harbin, 150086

Keywords:

Soybean;  PCR;  DN A isolation

PACS:

-

DOI:

10.11861/j.issn.1000-9841.1999.04.0318

Abstract:

The problem of grinding large numbers of small samples of f resh soy bean ti ssues rapidlyand w ithout loss w as solv ed by using a g rinder modified w ith a spiral ratchet screw driv er.The effect of different ex traction buffers, different temperatures and dif ferent t reatmenttimes on the quality of DN A w ere compared by ex amining the DN A with random- primerPCR. The result show ed that soy bean templet DN A suitable for PCR reaction could be gottenby using SDS ex traction buffer, 5 minutes treatment wi th 65- 80℃ and one time of extractionwith chloroform and iso- amy l alcohol. DN A was isola ted f rom mo re than a thousandsoybean samples wi th this procedure. The effect was stable.

References:

[1 ]　Hong Wang et al. 1993. A simple method of preparing plan t samples f or PCR. Nucleic Acids Res, 21( 7): 4153-4154

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