LIQUID FERMENTATION OF PHYTASE-PRODUCTION STEAIN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Author(s):: Zhang Meishen¹ Shen Ainguang² Xiong Xiaohui² Shen Chang²; 1 Hebei Academy of Agricultural and Forestry Science,Shijiazhuang,050051
2 Food Scientific and Technological College,Nanjing Agricalture University

Abstract:: With phytate-Ca as the sole phosphorus source of selected medium,a exeracellular phytase-producing strain was screened from the fiuum.Liquid fermentation was studied and a best fermentation medium was obtained,starch 8%,glucose 2%,NaNO31.0%,KH2PO40.01%,KCI0.05%,MgSO40.05%,FeSO40.01% and initial pH5.0.Inoculating maount 10%,filling liquid volum 10%,extracellular phytase production as much as 870.87pu/ml fermentation liquid was obtained at 30C for 90min.,and soluble protein content in treating liquid was increased from0.167mg/ml to 12.1mg/ml.

[1 ]　张梅申、熊晓辉、沈爱光, 1996,农牧产品开发, ( 11): 33- 34

[2 ]　Li lian U. Th ompson and Jan e H. yoon. J. 1984, Food Sci. , 49: 1228- 1229

[3 ]　尹光琳、战立克, 1992, 发酵工业全书,北京: 中国医药科技出版社, 534, 539

[4 ]　诸葛健、王正祥, 1994,工业微生物试验技术手册,北京: 中国轻工业出版社, 121- 214

[5 ]　陈祥照, 1957, 微生物通报, ( 7): 213

[6 ]　中国现场统计研究会农业优化组等, 1994,农业正交设计法,北京: 冶金工业出版社

[7 ]　WO94 /03612PC T /F193 /00310

[8 ]　(日)营原洁. 副岛正美著,张旭译, 1981,蛋白质的定量法,北京: 农业出版社, 186- 189

[9 ]　(英) F. G.普里斯特著, 1988,细胞外酶,北京: 科学出版社

[10 ]　吕耀昌、张玉良等著, 1990,农作物子粒中植酸盐离子交换测定方法的研究,作物品种资源, ( 2): 28- 31

Last Update: 2016-12-27