RAPD VERIFICATION OF THE SOYBEAN CULTIVAR "HEISHENG 101"(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Author(s):: Li Xichen　Lei Bo jun　Lu Cuihua　Qian Hua　Zho u Si jun　Han Yuqin　Liu Zhao jun; Bio. Res. Center, Heilongjiang Academy of Agri. Sci . , Harbin , 150086

Keywords:: Soy bean; Hei sheng 101; RAPD; Mo lecula r v eri ficatio n; Pollen tubecha nnel

Abstract:: Heisheng 101 w as the fi rst soybea n cul tiva r obtained f rom po llen tube cha nnel. Weused RAPD to ampli fy the to tal DN A of Heisheng 101 and it s dono r and recipient by 132ra ndom primers. The DN A polymo rphi sm resul ts of RAPD molecula r verificatio n indicated: Ei ther 3 sing le random primer or 2 do uble primers combina tions can be amplifiedpolymo rphic DN A f ragment s f rom Heisheng 101. The results proved that Hei sheng 101w as t ruly obtained f rom the t ransforma tion prog eny of it s do no r DN A fragment s int roducedinto recipient.

[1 ]　刘广阳等, 1996, 大豆科学, 15( 4): 353- 356

[2 ]　钱华等, 1997, 现代日用科学, ( 2): 40

[3 ]　黄骏麒等, 1986, 中国农业科学, ( 2): 16- 20

[4 ]　段晓岚等, 1985, 中国农业科学, ( 4): 6- 10

[5 ]　雷勃钧等, 1994, 中国科学B辑, 24( 6): 596- 601

[6 ]　K. M. Devos and M. D. Gale, 1992, Th eor Appl Genet , ( 84): 567- 572

[7 ]　Scot t V. Tingey et al. , 1993, Plant Ph ysi ol. , ( 101): 349- 352

[8 ]　L. Hart l et al. , 1995, Th eor Appl Genet , ( 90): 601- 606

[9 ]　M. S. Iqbal et al. , 1997, Theor Appl Genet , ( 94): 139- 144

[10 ]　惠东威等, 1992, 生物工程进展, 12( 6): 1- 5

[11 ]　李希臣等, 1994, 大豆科学, 13( 2): 153- 156

[12 ]　鲍晓明等, 1993,遗传学报, 20( 1): 81- 87

[13 ]　陈永强等, 1979,遗传, ( 1): 39- 40

[14 ]　王树林等, 1989,中国油料, ( 2): 63- 65

[15 ]　李希臣等, 1994,生物技术, 4( 3): 39- 41

[16 ]　J. Graham et al. , 1996, Th eor Appl Genet , ( 93): 402- 406

[17 ]　Bojh a kum p et al. , 1996, Plant Science, ( 114): 149- 158

[18 ]　孟安明, 1993, 生物工程进展, 14( 3): 46- 48

Last Update: 2016-12-27