THE PHYSIOLOGY CHANGES OF SOYBEANS AFIER TRANSFER OF ipt GENE INTO SOYBEAN BY Ti PLASMID AFTER INOCULAING WITH SMV NO.1(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

1999年03期

Page:

213-218

Research Field:

Publishing date:

Info

Title:

THE PHYSIOLOGY CHANGES OF SOYBEANS AFIER TRANSFER OF ipt GENE INTO SOYBEAN BY Ti PLASMID AFTER INOCULAING WITH SMV NO.1

Author(s):

Liu Lijun1 Wu Junjiang1 Gao Mingjie1 WangQingyin2 HuangYongfen2 Xia Kai3 Li Wenhua4

1 The Soybean Reseatch Institute,Heilongjiang Academy of Agricultwral Science

2 Department of Biology Harbin Normal University?

3 Naning Agricultural Universisy

4 Northeast Agricultural University

Keywords:

ipt gene;  Tramsformed plant;  SMV

PACS:

-

DOI:

10.11861/j.issn.1000-9841.1999.03.0213

Abstract:

Three cultivars of soybean of Heilongjiang Province have been transformed with iptgene into the soybean plants.The ELISA was used to study the endogenous hormonal amount and their balance condition and physiological change after the ipt gene being tramsformed into soybean plants.The results showed that the content of MDA became lower and the activity of SOD enzyme became higher after the tramsformation ipt into soybean plant cell by inoculation with SMV NO.1.The amount of endogenous IPA、IAA、GA,were higher in the soybean plant transformed with 8SL-pit and caused mophologicol change of the plant and increasing resistante to diseases of soybean.

References:

[1 ]　Barry. G. G. et al. , 1984, Identi fication of a cloned cytokinin bi osyn theti c gen e. Proc, Nat 1. Acad. Sci.V. S. A. 81: 4776- 4780

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