A PRELIMINARY STUDY ON TRANSFERRING CHITINASE GENE INTO SOYBEANS(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Author(s):: Xu Xiang ling　Zo u Lianpei　Liu Weihua　Li Jilin; Harbin Normal Univ ersity 150080

Abstract:: The chi tinase gene w as transferred into cultiva rs Do ng no ng 37, Ji lin 28, et al. w ithTi plasmid. Adventi tious nuds and reg ennera ted plant were inducted f rom co tylendonusnode a nd h ypoco tyl. PCR and DN A do t blo t ting prov ed tha t they w ere t ransferredplant. Puri fied plasmid PBin 437 with chitinase gene was also t ransferred into sev eralsoybeans va ridties by the pol len tube technique and 223 seeds were obtained. Among 113plants g ermina ted f rom these seeds 7 show ed positiv e reaction in PCR and DN A dotblo tting.

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Last Update: 2016-12-26