This peper presant s the studied results of a new analy sis method of t race totalf lav o noids in so ybean seeds. Zr4+ and flavo noids produced the f luorescet complex compound,and determined peak area integ ral v alue of fluo rescent spor ts o n filier pa per bymeans of T LC sca nning techno logy. The method ca n be applied as a quanti tativ e analysis to dif ferentiate f lav onoids compound f rom f lav onol. This method maybe a pplied effectively to study the forma fion mechanism o f seed coat mo t tling of soybea ns infected bySMV.