|Table of Contents|

PEG- MEDIATED TRANSFORMATION AND REGENERATION OFSOYBEAN PROTOPLAST WITH B ACI LLUS T HURIN GIEN SI S CrgIAc GENE(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
1998年04
Page:
326-330
Research Field:
Publishing date:

Info

Title:
PEG- MEDIATED TRANSFORMATION AND REGENERATION OFSOYBEAN PROTOPLAST WITH B ACI LLUS T HURIN GIEN SI S CrgIAc GENE
Author(s):
Nan Xiang ri　Liu Wenping　Liu Liyan　Lu Xiaobo　He Yunxia?Wei Zhiming
(B io. Res. Center , Heilongjiang Academy of Agri . Sci. Harbin , 150086)
( Shanghai Institute of Plant Physiology , Academia of Sinica , Shanghai , 200032)
Keywords:
Soy bean;  Baci llus thuringiensis Cry IAc gene;  Genome
PACS:
-
DOI:
10.11861/j.issn.1000-9841.1998.04.0326
Abstract:
Baci llus thuringiensis Cry IAc g ene was int roduced into the pro to plast of leadingsoybean cul tiv ars Heino ng 35, Heino ng 37, Hefeng 25 a nd Hefeng 35 wi th the PEGmethod. Through the screening wi th 30mg /l hyg romycin and the di fferentia tion of selectedresista nt calli, three reg enerated plants w ere obtained and t ransplanted successfully. PCR analy sis of the DN A f rom the transplanted plant s show ed posi tiv e reactio n.Southern blo t ana lysis of the PCR- posi tiv e pla nt s prov ed that the B. T Cry IAc g enehad integ ra ted into the g enome of these pla nt s.

References:

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