STUDY OF AFFECTING FACTORS IN SOYBEAN RAPD(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

1998年03

Page:

197-201

Research Field:

Publishing date:

Info

Title:

STUDY OF AFFECTING FACTORS IN SOYBEAN RAPD

Author(s):

Zou Jijun¹　Dong Wei²　Zhang Zhiyo ng²　Chen Shouyi²Wang Jichai³　Xu Jinxing¹　Yang Qingkai¹　Cao Yueping¹

1 Soybean Research Institute , Northeast Agricultural University , Harbin, 150030

2 Insti tute of Genetics, Academia Sinica , Beij ing , 100101,

3 Qiqihaer City Seed Bureau

Keywords:

RAPD;  Soy bean ( Gl ycine max ) ;  Affecting factors

PACS:

-

DOI:

10.11861/j.issn.1000-9841.1998.03.0197

Abstract:

To ensure tha t the results of RAPD w ill be reproducible and true, the ma jo r affectingfacto rs in soy bean RAPD were studied. These factors included: the co ncent ratio nso f Taq polymerase enzyme, Mg Cl2 , template, dN TPs, primer , amplificatio n o f pai red primers a nd thermal cycles. The final optimi zed soy bean RAPD co ndi tions w ere: in 25ulreactio n so lutio n, 10× Buf fer ( 100mM Tris- HCl pH8. 0, 500mM KCl , 0. 1% gelatin) ,2. 0mM MgCl2 , 0. 2mM dN TPs, 15ng primer, 1. 0u Taq polymerase enzyme. The results a lso show ed that compa red w ith the amplicatio n of single primer, paired primersno t o nly resul ted in mo re small f ragments but also lost some bands.

References:

[1 ]　Thomas H. J. , Stev er D. , Plant M ol ecular Biol og y Repor t, 1990, 8( 4): 297～ 303

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