STUDIES ON TRANSFERRING B. t. k- DELTA ENDOTOXINGENE INTO SOYBEAN WITH Ti- PLASMID PRIMARIL(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

1997年01

Page:

7-11

Research Field:

Publishing date:

Info

Title:

STUDIES ON TRANSFERRING B. t. k- DELTA ENDOTOXINGENE INTO SOYBEAN WITH Ti- PLASMID PRIMARIL

Author(s):

Xu Xiang ling　Gao Jing　Liu Weihua　Li Ji lin

( Harbin Normal Universi ty　150080)

Keywords:

So ybean;  Ti - plasmid;  B. t. k- del ta endo toxin g ene;  Reg eneratedplant

PACS:

-

DOI:

10.11861/j.issn.1000-9841.1997.01.0007

Abstract:

The pKT54 B7 C5 plasmid wi th B. t. k. - del ta endoxin gene w ere t rnsferred intoHeino ng 37 and Heino ng 39 et. al soy bean cultiva rs by inducting wi th v arious ex plantmethods. Adv enti tio us buds a nd regenera tion plant w ere obtained f rom hy pocotyl ,cotylendo n node. They were detected by kanamycine selectio n and alkaoids and primarilyprov ed that fo reig n gene were t ransferred into so ybean genome. Only 30 o f 81 regeneratedpla nt surviv ed. Only 3 plants wi th 7 pods w ere obtained f rom the surviv ed regeneratedplant s. It prov ed that 7 plants of the reg enerated plant s w ere t ra nsferred w ithfo rg ein g ene by PCR technique a nd dot hybri zatio n. The 7 seeds spro uted, plant g row this normal.

References:

[1 ]　田颖川等, 1991, “苏云金杆菌δ- 内毒素基因的转基因烟草的抗虫性”生物工程学报, 7( 1): 1- 10

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Memo:

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