ZHAO Jing-yun,MA Jun-kui,REN Xiao-jun,et al.Screening of Soybean Drought Resistance Phenotypic Index and Evaluation of Drought Resistant Resources[J].Soybean Science,2022,41(04):420-426.[doi:10.11861/j.issn.1000-9841.2022.04.0420]
大豆资源抗旱表型指标筛选及抗旱性评价
- Title:
- Screening of Soybean Drought Resistance Phenotypic Index and Evaluation of Drought Resistant Resources
- Keywords:
- soybean; drought stress; drought resistance identification; phenotype; indicator
- 文献标志码:
- A
- 摘要:
- 为探讨快捷鉴定大豆资源抗旱性的表型性状评价指标及评价方法,设置正常灌水和干旱处理,以黄淮海地区122份大豆资源为材料,分析干旱胁迫对大豆主要农艺性状的影响及其变化情况,筛选高效的大豆抗旱性评价表型指标性状。结果表明:干旱胁迫下,不同大豆资源的生物产量变异量最大,其次为单株粒数、株高、单株产量、单株荚数、生育期、百粒重和有效分枝数;前5个性状的变化量之和占8个性状变化总量的90.25%;干旱对各性状的抑制率分别为43.89%、43.29%、40.36%、39.66%、28.13%、19.70%、6.77%和4.02%。通过分析水旱两种条件下主要农艺性状的方差、变异系数、差值及抗旱指数,确定以产量、生物产量、单株荚数和株高4个性状作为抗旱鉴定的评价指标,为抗旱性评价提供了简捷有效的表型指标依据。以高度抗旱大豆品种晋豆21号为对照,基于这4个评价指标,计算标准抗旱指数,根据五级抗旱分类方法,鉴定出5份高度抗旱材料(汾豆92、石豆658、石豆111、阜02-1和周09013-1-1),及11份中高抗旱材料。
- Abstract:
- In order to explore the evaluation indexes and evaluation methods of phenotypic traits for rapid identification of drought resistance of soybean resources, we set up normal irrigation and drought treatments, used 122 soybean resources in Huang-Huai-Hai region to study the effects of drought stress on main agronomic characters of soybean and its variation, selection of high efficient drought resistance evaluation phenotypic index characters. The results showed that the variation of biomass yield was the largest under drought stress, followed by the seeds number per plant, plant height, yield per plant, pods number per plant, growth period, 100-seed weight and effective branching number. The total variation of the first five characters accounting for 90.25% of the total variation of the eight characters, and the inhibition rates of drought to the characters were 43.89%, 43.29%, 40.36%, 39.66%, 28.13%, 19.70%, 6.77% and 4.02%, respectively. Through the analysis of variance, coefficient of variation, difference and drought resistance index under the conditions of flood and drought, the yield, biological yield, pods number per plant and plant height were established as the evaluation indexes of drought resistance, which provided a simple and effective phenotypic index for drought resistance evaluation. In comparison with Jindou 21, based on these four evaluation indicators, calculated the standard drought resistance index, and according to the five-level drought resistance classification method, five high drought-resistant materials(Fendou 92, Shidou 658, Shidou 111, Fu 02-1 and Zhou 09013-1-1), and eleven middle-high drought-resistant varieties were evaluated by this comprehensive drought-resistant index method.
参考文献/References:
[1]胡荣海, 昌小平. 反复干旱法的生理基础及其应用[J]. 华北农学报, 1996, 11(3): 51-56. (HU R H, CHANG X P. The physiological base and utilization of repeated drought method[J]. Acta Agriculturae Boreali-Sinica, 1996, 11(3): 51-56.)[2]王瑞云, 王玉国, 养晓霞. 大豆抗旱的生理生态基础[J]. 山西农业大学学报, 2001, 21(3): 305-307. (WANG R R, WANG Y G, YANG X X. Physio-ecological foundation of drought-resistance of soybean[J]. Journal of Shanxi Agricultural University, 2001, 21(3): 305-307.)[3]刘学义. 干旱[M]//林汉明, 常汝镇, 邵桂花, 等. 中国大豆耐逆研究. 北京: 中国农业出版社, 2009: 1-60. (LIU X Y. Drought[M]//LAM H M, CHANG R Z, SHAO G H, et al. Tolerance research on tolerance to stresses in Chinese soybean. Beijing: China Agriculture Press, 2009: 1-60.)[4]蒲伟凤, 纪展波, 李桂兰, 等. 作物抗旱性鉴定方法研究进展[J]. 河北科技师范学院学报, 2011, 25(2): 34-39. (PU W F, JI Z B, LI G L, et al. Research progress on crop drought-resistance appraisal methods[J]. Journal of Hebei Normal University of Science and Technology, 2011, 25(2): 34-39.)[5]马俊奎, 刘小荣, 周伟, 等. 集水槽法在大豆抗旱性鉴定中的应用研究[J]. 大豆科学, 2016, 35(5): 766-771. (MA J K, LIU X R, ZHOU W, et al. Application of collecting trough method on identification of soybean drought resistance[J]. Soybean Science, 2016, 35(5): 766-771.)[6]祁旭升, 刘章雄, 关荣霞, 等. 大豆成株期抗旱性鉴定评价方法研究[J]. 作物学报, 2012, 38(4): 665-674. (QI X S, LIU Z X, GUAN R X, et al. Comparison of evaluation methods for drought-resistance at soybean adult stage[J]. Acta Agronomica Sinica, 2012, 38(4): 665-674.)[7]王敏, 张从宇, 马同富, 等. 大豆品种苗期抗旱性研究[J]. 中国油料作物学报, 2004, 26(3): 29-32. (WANG M, ZHANG C Y, MA T F, et al. Studies on the drought resistance of seedling in soybean[J]. Chinese Journal of Oil Crop Sciences, 2004, 26(3): 29-32.)[8]王燕平, 任海祥, 孙晓环, 等. 不同基因型大豆花荚期抗旱性综合评价[J]. 植物遗传资源学报, 2015, 16(1): 37-44. (WANG Y P, REN H X, SUN X H, et al. Comprehensive evaluation on drought resistance of different soybean cultivars at flowering-podding stage[J]. Journal of Plant Genetic Resources, 2015, 16(1): 37-44.)[9]王敏, 杨万明, 侯燕平, 等. 不同类型大豆花荚期抗旱性形态指标及其综合评价[J]. 核农学报, 2010, 24(1): 154-159. (WANG M, YANG W M, HOU Y P, et al. Morphological indexes of drought resistance of soybean accessions and its comprehensive evaluation on flowering and podding stage[J]. Journal of Nuclear Agricultural Sciences, 2010, 24(1): 154-159.)[10]邱丽娟, 常汝镇. 大豆种质资源描述规范和数据标准[M]. 北京: 中国农业版社, 2006. (QIU L J, CHANG R Z. Descriptors and data standard for soybean[M]. Beijing: China Agriculture Press, 2006.)[11]刘学义, 张小虎. 黄淮海地区大豆种质资源抗旱性鉴定及其研究[J]. 山西农业科学, 1993, 21(1): 19-24. (LIU X Y, ZHANG X H. Studies and evaluation on drought-tolerances of soybean germ plasm from Huang-Huai-Hai valleys[J]. Journal of Shanxi Agricultural Sciences, 1993, 21(1): 19-24.)[12]李贵全, 张海燕, 季兰, 等. 不同大豆品种抗旱性综合评价[J]. 应用生态学报, 2006, 17(12): 2408-2412. (LI G Q, ZHANG H Y, JI L, et al. Comprehensive evaluation on drought-resistance of different soybean varieties[J]. Chinese Journal of Applied Ecology, 2006, 17(12): 2408-2412.)[13]刘学义. 大豆抗旱性评定方法探[J]. 中国油料, 1986(4): 23-26. (LIU X Y. Evaluation method of drought resistance of soybean[J]. Chinese Oil, 1986(4): 23-26.)[14]乔亚科, 杨晓倩, 乔潇, 等. 大豆基于形态及生理指标的抗旱性评价及相关性分析[J]. 大豆科学, 2014, 33(5): 667-673. (QIAO Y K, YANG X Q, QIAO X, et al. The correlation of drought-resistance evaluation between agronomic traits and physiological indexes of wild and cultivated soybean[J]. Soybean Science, 2014, 33(5): 667-673.)[15]张小虎, 刘学义. 大豆品种资源抗旱性鉴定指标及方法[J]. 山西农业科学, 2011, 39(2): 106-108, 112. (ZHANG X H, LIU X Y. Drought resistance identification index and Methods in Soybean germ plasm[J]. Journal of Shanxi Agricultural Sciences, 2011, 39(2): 106-108, 112.)[16]张海燕,焦碧蝉,李贵全. 大豆抗旱性鉴定指标评价的研究[J]. 大豆科学, 2005,24(3): 183-188. (ZHANG H Y, JI B C, LI G Q. Study on selecting targets in drought resistance breeding of soybean[J]. Soybean Science, 2005, 24(3): 183-188.)[17]孙继颖, 高聚林, 薛春雷. 不同品种大豆抗旱性能比较研究[J]. 华北农学报, 2007, 22(6): 91-97. (SUN J Y, GAO J L, XUE C L. Comparative experiment on drought resistant characters of different soybean varieties[J]. Acta Agriculturae Boreali-Sinica, 2007, 22(6): 91-97.)[18]谢皓, 朱世明, 包子敬, 等. 干旱胁迫下大豆品种抗旱性评价与筛选[J]. 北京农学院学报, 2008, 23(3): 7-11. (XIE H, ZHU S M, BAO Z J, et al. Identification and selection of soybean drought resistant variety on the drought intimidation[J]. Journal of Beijing Agricultural University, 2008, 23(3): 7-11.)[19]汤复跃, 陈渊, 梁江, 等. 广西春大豆品种耐旱性鉴定研究[J]. 广西农学报, 2010, 25(6): 8-11. (TANG F Y, CHEN Y, LIANG J, et al. Study on identification in drought resistance of Guangxi spring soybean varieties[J]. Journal of Guangxi Agricultural, 2010, 25(6): 8-11.)[20]闫春娟, 宋书宏, 王文斌, 等. 大豆耐旱种质的鉴定[J]. 大豆科学, 2015, 34(1): 163-167. (YAN C J, SONG S H, WANG W B, et al. Identification of drought tolerans in soybean[J]. Soybean Science, 2015, 34(1): 163-167.)[21]潘相文, 李文滨, 李艳华, 等. 主成分分析在大豆抗旱性评价上的应用[J]. 大豆科学, 2006, 25(4): 379-389. (PAN X W, LI W B, LI Y H, et al. Application of principal component analysis in evaluation the drought-resistance of soybean genotypes[J]. Soybean Science, 2006, 25(4): 379-389.)[22]崔杰印, 武婷婷, 宋雯雯, 等. 黑龙江中上游地区早熟野生大豆种质资源的抗旱性鉴定[J]. 植物遗传资源学报, 2018, 19(6): 1073-1082. (CUI J Y, WU T T, SONG W W, et al. Drought tolerance evaluation of early-maturing annual wild soybean from the upper and middle reaches of the Heilongjiang River[J]. Journal of Plant Genetic Resources, 2018, 19(6): 1073-1082.)[23]王兴荣, 张彦军, 苟作旺, 等. 大豆种质资源抗旱性综合评价[J]. 干旱地区农业研究, 2015, 33(5): 17-23, 40. (WANG X R, ZHANG Y J, GOU Z W, et al. Comprehensive evaluation on drought-resistance traits of soybean germplasm resources[J]. Agricultural Research in the Arid Areas, 2015, 33(5): 17-23, 40.)[24]臧紫薇, 赵雪, 李海燕, 等. 大豆种质资源苗期抗旱性评价[J]. 大豆科学, 2016, 35(6): 964-968. (ZANG Z W, ZHAO X, LI H Y, et al. Evaluation of drought resistance of soybean germplasm in seedling stage[J]. Soybean Science, 2016, 35(6): 964-968.)[25]刘桂茹, 张荣芝, 卢建祥, 等. 小麦品种抗旱性鉴定指标与产量性状关系的探讨[J]. 河北农业大学学报, 1995, 18(1): 10-14. (LIU G R, ZHANG R Z, LU J X, et al. Relationship between yield and indices determining drought-resistance in winter wheat[J]. Journal of Hebei Agricultural University, 1995, 18(1): 10-14.)[26]景蕊莲. 作物抗旱研究的现状与思考[J]. 干旱地区农业研究, 1999, 17(2): 79-85. (JING R L. Present situation and approach of study on crop drought resistance[J]. Agricultural Research in the Arid Areas, 1999, 17(2): 79-85.)[27]SONGSRI P, JOGLOY S. KESMALA T, et al. Heritability of drought resistance traits and correlation of drought resistance and agronomic traits in peanut[J]. Crop Science, 2008, 48(6): 2245-2253.[28]胡福顺. 抗旱性鉴定[M]//李杏普.小麦遗传资源研究.北京: 中国农业大学出版社, 1997: 45-63. (HU F S. Drought resistance identification[M]//Li X P. Research on wheat genetic resources. Beijing: China Agricultural University Press, 1997: 45-63.) [29]谢成俊, 王平, 李卫民, 等. 小麦收获指数与主要农艺性状的相关性分析[J]. 中国农学通报, 2015, 31(3): 88-93. (XIE C J, WANG P, LI W M, et al. Correlation analysis between wheat harvest index and main agronomic characters[J]. Chinese Agricultural Science Bulletin, 2015, 31(3): 88-93.)[30]汪宝卿, 张礼凤, 戴海英, 等. 黄淮海地区夏大豆农业性状的遗传变异、相关及主成分分析[J]. 大豆科学, 2012, 31(2): 208-212. (WANG B Q, ZHANG L F, DAI H Y, et al. Genetic variation, correlation and principal component analysis on agronomic traits of summer sowing soybean(Glycine max Merr.) in Huanghuai Region[J]. Soybean science, 2012, 31(2): 208-212.)[31]李向华, 常汝镇. 中国春大豆品种聚类分析及主成分分析[J]. 作物学报, 1998, 24(3): 325-332. (LI X H, CHANG R Z. Cluster and principal component analysis of the spring soybean varieties in China[J]. Acta Agronomica Sinica, 1998, 24(3): 325-332.)[32]兰巨生. 农作物综合抗旱性的评价[J]. 中国农学通报, 1994, 10(5): 34-35. (LAN J S. Evaluation of comprehensive drought resistance of crops[J]. Chinese Agricultural Science Bulletin, 1994, 10(5): 34-35.)
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[17]刘丽君,林浩,唐晓飞,等.干旱胁迫对不同生育阶段大豆产量形态建成的影响[J].大豆科学,2011,30(03):405.[doi:10.11861/j.issn.1000-9841.2011.03.0405]
LIU Li-jun,LIN Hao,TANG Xiao-fei,et al.Drought Stress Influence Soybean Yield Morphogenesis in Different Growth Stages[J].Soybean Science,2011,30(04):405.[doi:10.11861/j.issn.1000-9841.2011.03.0405]
[18]赵坤,董守坤,刘丽君,等.干旱胁迫对春大豆开花期根系生理特性的影响[J].大豆科学,2010,29(03):437.[doi:10.11861/j.issn.1000-9841.2010.03.0437]
ZHAO Kun,DONG Shou-kun,LIU Li-jun,et al.Effects of Drought Stress on Physiological Characteristics of Root System of Spring Soybean in Flowering Period[J].Soybean Science,2010,29(04):437.[doi:10.11861/j.issn.1000-9841.2010.03.0437]
[19]李建英,田中艳,周长军,等.干旱胁迫下化控种衣剂对大豆幼苗生长发育及保护酶活性的影响[J].大豆科学,2010,29(04):611.[doi:10.11861/j.issn.1000-9841.2010.04.0611]
LI Jian-ying,TIAN Zhong-yan,ZHOU Chang-jun,et al.Effect of Chemical Seed Coating on Growth and Development of Soybean Seedlings under Drought Stress[J].Soybean Science,2010,29(04):611.[doi:10.11861/j.issn.1000-9841.2010.04.0611]
[20]钟鹏,吴俊江,刘丽君,等.低磷和干旱胁迫对不同基因型大豆光合生理特性的影响[J].大豆科学,2009,28(05):806.[doi:10.11861/j.issn.1000-9841.2009.05.0806]
ZHONG Peng,WU Jun-jiang,LIU Li-Jun,et al.Effects of Phosphorus Deficiency and Drought Stress on Photosynthetic Characters in Different Genotypic Soybeans[J].Soybean Science,2009,28(04):806.[doi:10.11861/j.issn.1000-9841.2009.05.0806]
备注/Memo
收稿日期:2022-02-05