SONG Jun-xia,QI Hong-bing,SU Xiao-ying.Antibacterial Activity of Bacillus natto[J].Soybean Science,2022,41(02):203-208.[doi:10.11861/j.issn.1000-9841.2022.02.0203]
纳豆芽胞杆菌抑菌活性研究
- Title:
- Antibacterial Activity of Bacillus natto
- 文献标志码:
- A
- 摘要:
- 为了研究纳豆芽胞杆菌高抑菌活性的最佳发酵条件，以苏云金芽孢杆菌和大肠杆菌为指示菌，采用滤纸片法，分别考察了培养时间、培养温度、培养基盐度、碳源、氮源及纳豆菌发酵粗提液浓度等因素对两种指示菌的抑制效果。结果表明：随着培养时间和粗提液浓度的提高，苏云金芽孢杆菌和大肠杆菌的抑菌圈直径先增后减；培养温度为37 ℃时苏云金芽孢杆菌的抑菌圈最大，42 ℃时大肠杆菌的抑菌圈最大；盐度为1%时，粗提液对苏云金芽孢杆菌和大肠杆菌的抑菌效果最好；碳源为牛肉膏时苏云金芽孢杆菌的抑菌圈最大，碳源为乳糖时大肠杆菌的抑菌圈最大；氮源为蛋白胨时苏云金芽孢杆菌的抑菌圈最大，氮源为硫酸铵时大肠杆菌的抑菌圈最大。正交法考察纳豆菌抑菌物质粗提液抑制两株指示菌的最优条件为培养时间36 h，粗提液浓度为100%，培养基盐度为1.5%。
- Abstract:
- In order to study the optimum fermentation conditions for the antibacterial activity of Bacillus natto, we took Bacillus thuringiensis and Escherichia coli as indicator bacterias and using filter paper method to study the inhibitory effect of antibacterial substance on two kinds of indicator bacteria about culture time， culture temperature， medium salinity， carbon source， nitrogen source and the concentration of the crude extract of natto antibacterial substance. The results showed that, With the increase of culture time and concentration of crude extract， the diameter of the inhibition zone of B. thuringiensis and E. coli increased firstly and then decreased.The incubation zone of B. thuringiensis was the largest at culture temperature of 37 ℃ and the inhibition zone of E. coli was the largest at 42 ℃. When the salinity was 1%， the inhibition zone of B. thuringiensis and E. coli were the largest. When the carbon source was beef extract， the inhibition zone of B. thuringiensis was the largest and when the carbon source was lactose， the largest inhibition zone of E. coli was the largest. The inhibition zone of B. thuringiensis was the largest when the nitrogen source was peptone， and the inhibition zone of E. coli was the largest when the nitrogen source was ammonium sulfate. The optimum conditions for the inhibition of crude extract of Bacillus natto to two indicator strains by orthogonal test were 36 h culture time, 100% concentration of crude extract and 1.5% salinity of medium.
参考文献/References:
［1］王笙戌，任宇杰，苑宁. 纳豆及其功能性质研究进展[J]. 粮食与油脂，2020，33（6）： 21-23.(WANG S X， REN Y J， YUAN N.Research progress on natto and its functional properties[J]. Cereals & Oils，2020，33（6）： 21-23.)[2]FUKUTAKE M. Quantification of genistein and genistinin beansand soybean products [J]. Food Chemical Toxicology， 1996， 34: 457-461.[3]SUMI H. Accumulation of vitamin k(menaquinone-7) in plasma after ingestion of natto and natto bacilli(B. subtilis natto) [J]. Food Science and Technology Research， 1999， 5(1): 48-50.[4]高研，张远龙，陈宁，等. 益生菌纳豆芽孢杆菌在动物饲料中的应用研究[J]. 中国饲料添加剂，2018（3）： 1-5.(GAO Y， ZHANG Y L， CHEN N， et al. Study on the application of probiotic Bacillus natto in animal feed[J] .China Feed Additive，2018（3）： 1-5.)[5]田海娟，张传智. 纳豆芽孢杆菌发酵食品的研究现状[J]. 食品安全导刊，2017（4）： 63. (TIAN H J， ZHANG C Z. Research status of Bacillus natto fermented food [J]. China Food Safety Magazine，2017（4）： 63.)[6]郭印，苌建菊，魏华， 等.饲料中添加纳豆芽孢杆菌对黄鳝生长的影响[J]. 安徽农学通报， 2019，25（14）： 69-70，91. (GUO Y， CHANG J J, WEI H，et al. The effects of Bacillus natto on growth of rice field eel[J]. Anhui Agricultural Science Bulletin， 2019，25（14）： 69-70，91.)[7]董小英， 唐胜球. 饲用纳豆菌生物学功能的研究进展 [J]. 中国畜牧杂志， 2012， 48(3): 63-66.（DONG X Q， TANG S Q. Progress on biological function of feed Bacillus natto[J]. Chinese Journal of Animal Science，2012， 48(3): 63-66.）[8]易灿， 蒋立文. 纳豆菌代谢产物抗菌作用的研究现状与展望 [J]. 农产品加工， 2008 (5): 64-67.(YI C， JIANG L W. Research status and prospect of antibacterial effect of natto metabolites [J]. Farm Products Processing， 2008 (5): 64-67.）[9]黄占旺， 魏萍， 刘海林，等. 纳豆菌生物学特性研究[J]. 江西农业大学学报， 2004， 6(1): 83-85. （HUANG Z W， WEI P， LIU H L， et al. A study on biological properties of Bacillus natto[J]. Acta Agriculturae Universitis Jiangxiensis， 2004， 6(1): 83-85.）[10]满丽莉， 向殿军. 纳豆菌培养条件的初步研究 [J]. 农产品加工， 2008 (8): 51-53. (MAN L L， XIANG D J. A preliminary study on cultural conditions of Bacillus natto[J]. Farm Products Processing， 2008 (8): 51-53.)[11]赵国萍，李迎秋，冯林慧，等. 天然防腐剂的应用研究进展[J].中国调味品，2017（8）： 155-159. (ZHAO G P， LI Y Q， FENG L H， et al. Research progress on application of natural preservatives[J]. China Condiment，2017（8）： 155-159.)[12]邢海丽， 辛嘉英， 王艳， 等. 微生物源天然食品防腐剂的研究进展[J]. 食品安全质量检测学报， 2015， 6(10): 3890-3894.（XING H L， XING J Y，WANG Y， et al. Research progress of natural food preservatives from microbial source[J]. Journal of Food Safety & Quality， 2015， 6(10): 3890-3894.）[13]张丽靖， 杨郁. 纳豆菌作为食品防腐剂的研究进展 [J]. 食品研究与开发， 2008， 29(5): 188-190.(ZHANG L J， YANG Y. Progress to Bacillus natto as food preservative [J]. Food Reserch and Development， 2008， 29(5): 188-190.)[14]南芝润，侯磊，任莹，等.纳豆及其产物的研究与应用[J]. 山西农业科学， 2017， 45(10): 1721-1724，1736. (NAN Z R， HOU L， REN Y， et al. Study and application of natto and its products[J]. Journal of Shanxi Agricultural Sciences， 2017， 45(10): 1721-1724，1736.)[15]王继伟， 葛英亮， 于晋， 等. 纳豆芽抱杆菌次生代谢产物抑菌功能的研究 [J]. 食品与机械， 2013， 29(3): 94-100.(WANG J W， GE Y L， YU J， et al. Study on bacteriostatic effect of Bacillus natto metabolic product [J]. Food & Machinery， 2013， 29(3): 94-100.)[16]祁红兵， 宋军霞， 张卫. 纳豆芽孢杆菌固体发酵麸皮的抗氧化功能研究 [J]. 粮食与饲料工业， 2014 (5): 51-56.(QI H B， SONG J X， ZHANG W. Antioxidant function of wheat bran solid fermentation by Bacillus natto[J]. Cereal & Feed Industry， 2014 (5): 51-56.)[17]钟青萍， 谢俊杰， 余世望. 纳豆菌的分离鉴定及其抗菌活性 [J]. 食品科学， 2002 (10): 109-112.(ZHONG Q P， XIE J J， YU S W. Isolation，identification and antimicrobial activity assay of Bacillus natto[J]. Food Science，2002(10): 109-112.)[18]付志英，刘彩珍，周红艳，等. 纳豆菌拮抗黄曲霉试验方法的研究[J]. 中国调味品，2019， 44(4)：93-96.（FU Z Y， LIU C Z， ZHOU H Y， et al. Study on the method of antagonizing aspergillus flavus by Bacillus natto[J]. China Condiment， 2019， 44(4)：93-96.）[19]王青云， 林亲录， 彭宽， 等. 纳豆芽孢杆菌Bna05菌株产抗霉脂肽的鉴定[J].微生物学通报， 2017(11): 2660-2668.(WANG Q Y， LIN Q L ， PENG K， et al. Isolate and identify lipopeptides in metabolites of Bacillus subtilis subsp.natto Bna05[J]. Microbiology， 2017 (11): 2660-2668.)[20]陈庆， 昝航. 一种用于高效水处理的抗菌复合反渗透膜及制备方法:中国， CN201810192846.8[P]. 2018-03-09.(CHEN Q， MIAN H. The invention relates to an antibacterial composite reverse osmosis membrane for high efficiency water treatment and a preparation method thereof: China， CN201810192846.8[P]. 2018-3-9.)[21]李津津， 王伟， 陶乐仁， 等. 枯草杆菌素Subtillicin L的纯化鉴定及抑菌性质[J]. 食品与发酵工业， 2020， 46 (18): 8-12.(LI J J， WANG W， TAO L R， et al. Purification， identification and antimicrobial characteristics of Subtillicin L produced by Bacillus subtillis DH8043[J]. Food and Fermentation Industries， 2020， 46 (18): 8-12.)
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备注/Memo
收稿日期：2021-08-21