CHEN Feng-qiong,CHEN Qiu-sen,LIU Han-lin,et al.Regulatory Effects of Different Exogenous Reagent on Vegetable Soybean Under Low Temperature Stress[J].Soybean Science,2022,41(02):165-171.[doi:10.11861/j.issn.1000-9841.2022.02.0165]
不同外源试剂对菜用大豆低温胁迫的调控效应
- Title:
- Regulatory Effects of Different Exogenous Reagent on Vegetable Soybean Under Low Temperature Stress
- Keywords:
- vegetable soybean; chilling; exogenous reagent; gibberellin; γ-aminobutyric acid
- 文献标志码:
- A
- 摘要:
- 为了筛选可以有效缓解菜用大豆低温胁迫伤害的外源试剂，并明确其对菜用大豆低温胁迫的调控效应。本试验以菜用大豆品种辽鲜1号为供试品种，以赤霉素、乙烯利、阿魏酸、γ-氨基丁酸、壳聚糖和绿原酸为供试试剂，比较外源试剂对低温胁迫下菜用大豆的抗氧化系统、渗透调节物质和氮代谢的影响，筛选能够增强菜用大豆低温胁迫耐性的外源试剂。结果表明：外源GA3、GABA处理显著提高了低温胁迫下菜用大豆的抗氧化酶活性，尤其是SOD和CAT活性，并显著提高了AsA和GSH含量，显著降低了超氧阴离子（O-2）含量，缓解了菜用大豆受到的氧化损伤。此外，外源GA3、GABA处理使菜用大豆可溶性糖和蔗糖含量显著增加，增强其细胞保水能力；同时显著提高了菜用大豆氮代谢酶活性，尤其是硝酸还原酶(NR)、谷氨酸合酶（GOGAT）和谷氨酸脱氢酶（GDH）活性，促进菜用大豆氮代谢作用，增强其低温胁迫耐性。由此可知，外源GA3和GABA处理通过调节抗氧化性、渗透调节物质和氮代谢来缓解菜用大豆的耐冷能力。
- Abstract:
- In order to select the exogenous reagents that can effectively alleviate the injury of vegetable soybean, and clarify its regulatory effect on vegetable soybean under low temperature stress, the vegetable soybean variety Liaoxian 1 was used as the test variety, and compared the effects of gibberellin, ethephon, ferulic acid γ-aminobutyric acid, chitosan and chlorogenic acid on antioxidant system, osmoregulation substances and nitrogen metabolism of vegetable soybean under low temperature stress. The results showed that exogenous GA3 and GABA treatment significantly increased the antioxidant enzyme activities, especially the activities of SOD and CAT, significantly increased the contents of ASA and GSH, significantly reduced the content of superoxide anion (O-2) and alleviated the oxidative damage of vegetable soybean under low temperature stress. In addition, exogenous GA3 and GABA treatment significantly increased the soluble sugar and sucrose content of vegetable soybean and enhanced its cell water holding capacity. At the same time, it significantly improved the activities of nitrogen metabolism enzymes in vegetable soybean, especially the activities of nitrate reductase (NR), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH), promoted the nitrogen metabolism of vegetable soybean and enhanced the tolerance to low temperature stress. It can be seen that exogenous GA3 and GABA treatment can alleviate the cold tolerance of vegetable soybean by regulating antioxidant, osmotic regulatory substances and nitrogen metabolism.
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备注/Memo
收稿日期：2021-08-15