WANG Xiao-li,WANG Min,YUE Ai-qin,et al.Genome-Wide Identification of Soybean TGL Gene Family and The Response to High Salt Stress[J].Soybean Science,2022,41(01):12-019.[doi:10.11861/j.issn.1000-9841.2022.01.0012]
大豆TGL基因家族全基因组鉴定及高盐胁迫响应研究
- Title:
- Genome-Wide Identification of Soybean TGL Gene Family and The Response to High Salt Stress
- 文献标志码:
- A
- 摘要:
- 三酰甘油脂肪酶（TGL）是甘油三脂分解代谢的主要酶，TGLs能水解参与植物生长发育和非生物胁迫反应的长链脂肪酸甘油三酯（TAG）。为研究大豆TGL基因家族的基因成员及其对高盐胁迫的响应，本研究通过同源比对方法在大豆中鉴定GmTGLs基因并对其进行生物信息学分析，根据RNA-seq数据库并采用荧光定量PCR方法分析盐胁迫下基因的表达量差异，并对主要盐胁迫响应基因进行生物信息学分析。结果显示：共从大豆中鉴定出15个GmTGLs基因，不均匀地分布在11条染色体上，其编码氨基酸序列为392~701 aa。这些GmTGLs均有保守结构域Abhydro_lipase(PF04083)。启动子顺式作用元件分析表明，GmTGLs的启动子包含光响应顺式元件，激素响应元件及ARE、LTR、TC-rich repeats和MBS等胁迫响应元件。转录组数据分析表明GmTGL3、GmTGL14基因在所有组织中均有表达，GmTGL3、GmTGL14、GmTGL2和GmTGL6基因在盐胁迫后表达上调，GmTGL2和GmTGL3基因的表达变化最明显。荧光定量PCR分析结果表明GmTGL2和GmTGL3可能分别主要在盐胁迫的后期和前期参与响应，进一步证实TGL基因家族参与了大豆对盐胁迫的反应过程。
- Abstract:
- Triacylglycerol lipase (TGL) is the main enzyme of triglyceride catabolism. TGLs can hydrolyze long-chain fatty acid triglycerides involved in plant growth, development and abiotic stress response. In order to study the TGL gene family members in soybean and their response to high salt stress， this study identified GmTGL genes in soybean， and analyzed the physicochemical properties， systematic evolution， gene structure， conserved domain， chromosome location and tissue expression pattern based on bioinformatics methods. We analyzed the differences of gene expression under salt stress based on the RNA-seq database and the fluorescence quantitative PCR method. The results showed that a total of 15 GmTGL genes were identified from soybean， their encoding amino acid sequences ranged from 392 to 701 aa， and the GmTGL genes was unevenly distributed on 11 chromosomes. These GmTGLs all shared a common conserved domain Abhydro_lipase(PF04083). The promoter analysis showed that the promoter of GmTGLs contained cis-responsive elements， hormone responsive elements， and stress responsive elements such as ARE， LTR， TC-rich Repeats and MBS. GmTGL3 and GmTGL14 were expressed in all soybean tissues， and the expressions of GmTGL3， GmTGL14， GmTGL2 and GmTGL6 were up-regulated in response to salt stress. GmTGL2 and GmTGL3 genes with the most obvious up-regulated expression under salt stress were selected for semi-quantitative analysis， which was further verified that TGL gene family could participate in the response process of soybean to salt stress.
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备注/Memo
收稿日期：2021-09-30